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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Roseiflexus castenholzii Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP415834RIP
CSB-EP415834RIP
CSB-BP415834RIP
CSB-MP415834RIP
CSB-EP415834RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP415835RIP
CSB-EP415835RIP
CSB-BP415835RIP
CSB-MP415835RIP
CSB-EP415835RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii ATP synthase gamma chain (atpG) CSB-YP415836RIP
CSB-EP415836RIP
CSB-BP415836RIP
CSB-MP415836RIP
CSB-EP415836RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP415837RIP
CSB-EP415837RIP
CSB-BP415837RIP
CSB-MP415837RIP
CSB-EP415837RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Elongation factor P (efp) CSB-YP415838RIP
CSB-EP415838RIP
CSB-BP415838RIP
CSB-MP415838RIP
CSB-EP415838RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Ribosome maturation factor RimM (rimM) CSB-YP415839RIP
CSB-EP415839RIP
CSB-BP415839RIP
CSB-MP415839RIP
CSB-EP415839RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP415840RIP
CSB-EP415840RIP
CSB-BP415840RIP
CSB-MP415840RIP
CSB-EP415840RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii NADH-quinone oxidoreductase subunit N 1 (nuoN1), partial CSB-YP415841RIP
CSB-EP415841RIP
CSB-BP415841RIP
CSB-MP415841RIP
CSB-EP415841RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L19 (rplS) CSB-YP415842RIP
CSB-EP415842RIP
CSB-BP415842RIP
CSB-MP415842RIP
CSB-EP415842RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP415843RIP
CSB-EP415843RIP
CSB-BP415843RIP
CSB-MP415843RIP
CSB-EP415843RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP415844RIP
CSB-EP415844RIP
CSB-BP415844RIP
CSB-MP415844RIP
CSB-EP415844RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP415845RIP
CSB-EP415845RIP
CSB-BP415845RIP
CSB-MP415845RIP
CSB-EP415845RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP415846RIP
CSB-EP415846RIP
CSB-BP415846RIP
CSB-MP415846RIP
CSB-EP415846RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP415847RIP
CSB-EP415847RIP
CSB-BP415847RIP
CSB-MP415847RIP
CSB-EP415847RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP415848RIP
CSB-EP415848RIP
CSB-BP415848RIP
CSB-MP415848RIP
CSB-EP415848RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L15 (rplO) CSB-YP415849RIP
CSB-EP415849RIP
CSB-BP415849RIP
CSB-MP415849RIP
CSB-EP415849RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L5 (rplE) CSB-YP415850RIP
CSB-EP415850RIP
CSB-BP415850RIP
CSB-MP415850RIP
CSB-EP415850RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 30S ribosomal protein S19 (rpsS) CSB-YP415851RIP
CSB-EP415851RIP
CSB-BP415851RIP
CSB-MP415851RIP
CSB-EP415851RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 30S ribosomal protein S7 (rpsG) CSB-YP415852RIP
CSB-EP415852RIP
CSB-BP415852RIP
CSB-MP415852RIP
CSB-EP415852RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Heat-inducible transcription repressor HrcA (hrcA) CSB-YP415853RIP
CSB-EP415853RIP
CSB-BP415853RIP
CSB-MP415853RIP
CSB-EP415853RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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