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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus 50S ribosomal protein L22 (rplV) CSB-YP416674SUK
CSB-EP416674SUK
CSB-BP416674SUK
CSB-MP416674SUK
CSB-EP416674SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L4 (rplD) CSB-YP416675SUK
CSB-EP416675SUK
CSB-BP416675SUK
CSB-MP416675SUK
CSB-EP416675SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein FdhD homolog (fdhD) CSB-YP416676SUK
CSB-EP416676SUK
CSB-BP416676SUK
CSB-MP416676SUK
CSB-EP416676SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithorhynchus anatinus Large proline-rich protein BAG6 (BAG6), partial CSB-YP416677OEX
CSB-EP416677OEX
CSB-BP416677OEX
CSB-MP416677OEX
CSB-EP416677OEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP416678SUK
CSB-EP416678SUK
CSB-BP416678SUK
CSB-MP416678SUK
CSB-EP416678SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Oxygen sensor histidine kinase nreB (nreB) CSB-YP416679SUK
CSB-EP416679SUK
CSB-BP416679SUK
CSB-MP416679SUK
CSB-EP416679SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thrasops jacksonii Toxin 3FTx-Thr5 CSB-YP416680TKG
CSB-EP416680TKG
CSB-BP416680TKG
CSB-MP416680TKG
CSB-EP416680TKG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP416681SUK
CSB-EP416681SUK
CSB-BP416681SUK
CSB-MP416681SUK
CSB-EP416681SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus L-lactate dehydrogenase 2 (ldh2) CSB-YP416682SUK
CSB-EP416682SUK
CSB-BP416682SUK
CSB-MP416682SUK
CSB-EP416682SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP416683SUK
CSB-EP416683SUK
CSB-BP416683SUK
CSB-MP416683SUK
CSB-EP416683SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0397 protein SAHV_2669 (SAHV_2669), partial CSB-YP416684SUK1
CSB-EP416684SUK1
CSB-BP416684SUK1
CSB-MP416684SUK1
CSB-EP416684SUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease P protein component (rnpA) CSB-YP416685SUK
CSB-EP416685SUK
CSB-BP416685SUK
CSB-MP416685SUK
CSB-EP416685SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Torque teno virus ORF2/4 protein (ORF2/4) CSB-YP416686TKJ
CSB-EP416686TKJ
CSB-BP416686TKJ
CSB-MP416686TKJ
CSB-EP416686TKJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable beta-1,3-galactosyltransferase 20 (B3GALT20), partial CSB-YP416687DOA1
CSB-EP416687DOA1
CSB-BP416687DOA1
CSB-MP416687DOA1
CSB-EP416687DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Selection and upkeep of intraepithelial T-cells protein 2 (Skint2), partial CSB-YP416688MO1
CSB-EP416688MO1
CSB-BP416688MO1
CSB-MP416688MO1
CSB-EP416688MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP416689YAN
CSB-EP416689YAN
CSB-BP416689YAN
CSB-MP416689YAN
CSB-EP416689YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b UPF0207 protein YpsIP31758_1449 (YpsIP31758_1449) CSB-YP416690YAN
CSB-EP416690YAN
CSB-BP416690YAN
CSB-MP416690YAN
CSB-EP416690YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP416691YAN
CSB-EP416691YAN
CSB-BP416691YAN
CSB-MP416691YAN
CSB-EP416691YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b N-methyl-L-tryptophan oxidase (solA) CSB-YP416692YAN
CSB-EP416692YAN
CSB-BP416692YAN
CSB-MP416692YAN
CSB-EP416692YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Beta-hexosaminidase (nagZ) CSB-YP416693YAN
CSB-EP416693YAN
CSB-BP416693YAN
CSB-MP416693YAN
CSB-EP416693YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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