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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus tRNA pseudouridine synthase B (truB) CSB-YP416634SUK
CSB-EP416634SUK
CSB-BP416634SUK
CSB-MP416634SUK
CSB-EP416634SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA mismatch repair protein MutS (mutS), partial CSB-YP416635SUK
CSB-EP416635SUK
CSB-BP416635SUK
CSB-MP416635SUK
CSB-EP416635SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L33 1 (rpmG1) CSB-YP416636SUK
CSB-EP416636SUK
CSB-BP416636SUK
CSB-MP416636SUK
CSB-EP416636SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Nuclease sbcCD subunit C (sbcC), partial CSB-YP416637SUK
CSB-EP416637SUK
CSB-BP416637SUK
CSB-MP416637SUK
CSB-EP416637SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Indole-3-glycerol phosphate synthase (trpC) CSB-YP416638SUK
CSB-EP416638SUK
CSB-BP416638SUK
CSB-MP416638SUK
CSB-EP416638SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acylphosphatase (acyP) CSB-YP416639SUK
CSB-EP416639SUK
CSB-BP416639SUK
CSB-MP416639SUK
CSB-EP416639SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction resolvase recU (recU) CSB-YP416640SUK
CSB-EP416640SUK
CSB-BP416640SUK
CSB-MP416640SUK
CSB-EP416640SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chorismate synthase (aroC) CSB-YP416641SUK
CSB-EP416641SUK
CSB-BP416641SUK
CSB-MP416641SUK
CSB-EP416641SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Segregation and condensation protein B (scpB) CSB-YP416642SUK
CSB-EP416642SUK
CSB-BP416642SUK
CSB-MP416642SUK
CSB-EP416642SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus N utilization substance protein B homolog (nusB) CSB-YP416643SUK
CSB-EP416643SUK
CSB-BP416643SUK
CSB-MP416643SUK
CSB-EP416643SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Shikimate kinase (aroK) CSB-YP416644SUK
CSB-EP416644SUK
CSB-BP416644SUK
CSB-MP416644SUK
CSB-EP416644SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S21 (rpsU) CSB-YP416645SUK
CSB-EP416645SUK
CSB-BP416645SUK
CSB-MP416645SUK
CSB-EP416645SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor 4 (lepA), partial CSB-YP416646SUK
CSB-EP416646SUK
CSB-BP416646SUK
CSB-MP416646SUK
CSB-EP416646SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcription elongation factor GreA (greA) CSB-YP416647SUK
CSB-EP416647SUK
CSB-BP416647SUK
CSB-MP416647SUK
CSB-EP416647SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartate--tRNA ligase (aspS), partial CSB-YP416648SUK
CSB-EP416648SUK
CSB-BP416648SUK
CSB-MP416648SUK
CSB-EP416648SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP416649SUK
CSB-EP416649SUK
CSB-BP416649SUK
CSB-MP416649SUK
CSB-EP416649SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP416650SUK
CSB-EP416650SUK
CSB-BP416650SUK
CSB-MP416650SUK
CSB-EP416650SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Trigger factor (tig) CSB-YP416651SUK
CSB-EP416651SUK
CSB-BP416651SUK
CSB-MP416651SUK
CSB-EP416651SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP416652SUK
CSB-EP416652SUK
CSB-BP416652SUK
CSB-MP416652SUK
CSB-EP416652SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tyrosine--tRNA ligase (tyrS) CSB-YP416653SUK
CSB-EP416653SUK
CSB-BP416653SUK
CSB-MP416653SUK
CSB-EP416653SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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