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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus subsp. cytotoxis Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP417434BOK
CSB-EP417434BOK
CSB-BP417434BOK
CSB-MP417434BOK
CSB-EP417434BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Probable tRNA sulfurtransferase (thiI) CSB-YP417435BOK
CSB-EP417435BOK
CSB-BP417435BOK
CSB-MP417435BOK
CSB-EP417435BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP417436BOK
CSB-EP417436BOK
CSB-BP417436BOK
CSB-MP417436BOK
CSB-EP417436BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 2-succinylbenzoate--CoA ligase (menE) CSB-YP417437BOK
CSB-EP417437BOK
CSB-BP417437BOK
CSB-MP417437BOK
CSB-EP417437BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Ferredoxin--NADP reductase 2 (Bcer98_3539) CSB-YP417438BOK
CSB-EP417438BOK
CSB-BP417438BOK
CSB-MP417438BOK
CSB-EP417438BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Phosphoglycerate kinase (pgk) CSB-YP417439BOK
CSB-EP417439BOK
CSB-BP417439BOK
CSB-MP417439BOK
CSB-EP417439BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP417440BOK1
CSB-EP417440BOK1
CSB-BP417440BOK1
CSB-MP417440BOK1
CSB-EP417440BOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Uracil phosphoribosyltransferase (upp) CSB-YP417441BOK
CSB-EP417441BOK
CSB-BP417441BOK
CSB-MP417441BOK
CSB-EP417441BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP417442BOK
CSB-EP417442BOK
CSB-BP417442BOK
CSB-MP417442BOK
CSB-EP417442BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP417443CWB
CSB-EP417443CWB
CSB-BP417443CWB
CSB-MP417443CWB
CSB-EP417443CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Phosphoglucosamine mutase (glmM) CSB-YP417444CWB
CSB-EP417444CWB
CSB-BP417444CWB
CSB-MP417444CWB
CSB-EP417444CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP417445CWB
CSB-EP417445CWB
CSB-BP417445CWB
CSB-MP417445CWB
CSB-EP417445CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP417446CWB
CSB-EP417446CWB
CSB-BP417446CWB
CSB-MP417446CWB
CSB-EP417446CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP417447CWB
CSB-EP417447CWB
CSB-BP417447CWB
CSB-MP417447CWB
CSB-EP417447CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP417448CWB
CSB-EP417448CWB
CSB-BP417448CWB
CSB-MP417448CWB
CSB-EP417448CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP417449CWB
CSB-EP417449CWB
CSB-BP417449CWB
CSB-MP417449CWB
CSB-EP417449CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Probable transcriptional regulatory protein Ccur92_05350 (Ccur92_05350) CSB-YP417450CWB
CSB-EP417450CWB
CSB-BP417450CWB
CSB-MP417450CWB
CSB-EP417450CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP417451CWB
CSB-EP417451CWB
CSB-BP417451CWB
CSB-MP417451CWB
CSB-EP417451CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP417452CWB
CSB-EP417452CWB
CSB-BP417452CWB
CSB-MP417452CWB
CSB-EP417452CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus tRNA (cmo5U34)-methyltransferase CSB-YP417453CWB
CSB-EP417453CWB
CSB-BP417453CWB
CSB-MP417453CWB
CSB-EP417453CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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