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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio harveyi Probable cytosol aminopeptidase (pepA), partial CSB-YP417734VEA
CSB-EP417734VEA
CSB-BP417734VEA
CSB-MP417734VEA
CSB-EP417734VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Phosphopantetheine adenylyltransferase (coaD) CSB-YP417735VEA
CSB-EP417735VEA
CSB-BP417735VEA
CSB-MP417735VEA
CSB-EP417735VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi High frequency lysogenization protein HflD homolog (hflD) CSB-YP417736VEA
CSB-EP417736VEA
CSB-BP417736VEA
CSB-MP417736VEA
CSB-EP417736VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Arginine--tRNA ligase (argS), partial CSB-YP417737VEA
CSB-EP417737VEA
CSB-BP417737VEA
CSB-MP417737VEA
CSB-EP417737VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Copper homeostasis protein CutC (cutC) CSB-YP417738VEA
CSB-EP417738VEA
CSB-BP417738VEA
CSB-MP417738VEA
CSB-EP417738VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP417739VEA
CSB-EP417739VEA
CSB-BP417739VEA
CSB-MP417739VEA
CSB-EP417739VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Protein sprT (sprT) CSB-YP417740VEA
CSB-EP417740VEA
CSB-BP417740VEA
CSB-MP417740VEA
CSB-EP417740VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP417741VEA
CSB-EP417741VEA
CSB-BP417741VEA
CSB-MP417741VEA
CSB-EP417741VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Der GTPase-activating protein YihI (yihI) CSB-YP417742VEA
CSB-EP417742VEA
CSB-BP417742VEA
CSB-MP417742VEA
CSB-EP417742VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Co-chaperone protein hscB homolog (hscB) CSB-YP417743VEA
CSB-EP417743VEA
CSB-BP417743VEA
CSB-MP417743VEA
CSB-EP417743VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi UPF0133 protein VIBHAR_03086 (VIBHAR_03086) CSB-YP417744VEA
CSB-EP417744VEA
CSB-BP417744VEA
CSB-MP417744VEA
CSB-EP417744VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Cytidylate kinase (cmk) CSB-YP417745VEA
CSB-EP417745VEA
CSB-BP417745VEA
CSB-MP417745VEA
CSB-EP417745VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Peptide chain release factor 3 (prfC), partial CSB-YP417746VEA
CSB-EP417746VEA
CSB-BP417746VEA
CSB-MP417746VEA
CSB-EP417746VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP417747VEA
CSB-EP417747VEA
CSB-BP417747VEA
CSB-MP417747VEA
CSB-EP417747VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Electron transport complex protein RnfA (rnfA), partial CSB-YP417748VEA1
CSB-EP417748VEA1
CSB-BP417748VEA1
CSB-MP417748VEA1
CSB-EP417748VEA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP417749VEA
CSB-EP417749VEA
CSB-BP417749VEA
CSB-MP417749VEA
CSB-EP417749VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP417750VEA
CSB-EP417750VEA
CSB-BP417750VEA
CSB-MP417750VEA
CSB-EP417750VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L30 (rpmD) CSB-YP417751VEA
CSB-EP417751VEA
CSB-BP417751VEA
CSB-MP417751VEA
CSB-EP417751VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 50S ribosomal protein L23 (rplW) CSB-YP417752VEA
CSB-EP417752VEA
CSB-BP417752VEA
CSB-MP417752VEA
CSB-EP417752VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP417753VEA
CSB-EP417753VEA
CSB-BP417753VEA
CSB-MP417753VEA
CSB-EP417753VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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