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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. holarctica NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP417814FDV
CSB-EP417814FDV
CSB-BP417814FDV
CSB-MP417814FDV
CSB-EP417814FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Potassium-transporting ATPase A chain (kdpA), partial CSB-YP417815FDV
CSB-EP417815FDV
CSB-BP417815FDV
CSB-MP417815FDV
CSB-EP417815FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Recombination protein RecR (recR) CSB-YP417816RIP
CSB-EP417816RIP
CSB-BP417816RIP
CSB-MP417816RIP
CSB-EP417816RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Tyrosine recombinase XerC (xerC) CSB-YP417817RIP
CSB-EP417817RIP
CSB-BP417817RIP
CSB-MP417817RIP
CSB-EP417817RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii UPF0753 protein Rcas_0567 (Rcas_0567), partial CSB-YP417818RIP
CSB-EP417818RIP
CSB-BP417818RIP
CSB-MP417818RIP
CSB-EP417818RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Cytidylate kinase (cmk) CSB-YP417819RIP
CSB-EP417819RIP
CSB-BP417819RIP
CSB-MP417819RIP
CSB-EP417819RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta 1 (accD1) CSB-YP417820RIP
CSB-EP417820RIP
CSB-BP417820RIP
CSB-MP417820RIP
CSB-EP417820RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L20 (rplT) CSB-YP417821RIP
CSB-EP417821RIP
CSB-BP417821RIP
CSB-MP417821RIP
CSB-EP417821RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (bchL) CSB-YP417822RIP
CSB-EP417822RIP
CSB-BP417822RIP
CSB-MP417822RIP
CSB-EP417822RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP417823RIP
CSB-EP417823RIP
CSB-BP417823RIP
CSB-MP417823RIP
CSB-EP417823RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii 50S ribosomal protein L21 (rplU) CSB-YP417824RIP
CSB-EP417824RIP
CSB-BP417824RIP
CSB-MP417824RIP
CSB-EP417824RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Phosphoglycerate kinase (pgk) CSB-YP417825RIP
CSB-EP417825RIP
CSB-BP417825RIP
CSB-MP417825RIP
CSB-EP417825RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP417826RIP
CSB-EP417826RIP
CSB-BP417826RIP
CSB-MP417826RIP
CSB-EP417826RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Ribonuclease HII (rnhB) CSB-YP417827RIP
CSB-EP417827RIP
CSB-BP417827RIP
CSB-MP417827RIP
CSB-EP417827RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Protein RecA (recA) CSB-YP417828RIP
CSB-EP417828RIP
CSB-BP417828RIP
CSB-MP417828RIP
CSB-EP417828RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Transcriptional repressor NrdR (nrdR) CSB-YP417829RIP
CSB-EP417829RIP
CSB-BP417829RIP
CSB-MP417829RIP
CSB-EP417829RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii NADH-quinone oxidoreductase subunit N 2 (nuoN2), partial CSB-YP417830RIP
CSB-EP417830RIP
CSB-BP417830RIP
CSB-MP417830RIP
CSB-EP417830RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP417831RIP
CSB-EP417831RIP
CSB-BP417831RIP
CSB-MP417831RIP
CSB-EP417831RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Chorismate synthase (aroC) CSB-YP417832RIP
CSB-EP417832RIP
CSB-BP417832RIP
CSB-MP417832RIP
CSB-EP417832RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP417833RIP
CSB-EP417833RIP
CSB-BP417833RIP
CSB-MP417833RIP
CSB-EP417833RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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