Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Cronobacter sakazakii Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP417694CXL
CSB-EP417694CXL
CSB-BP417694CXL
CSB-MP417694CXL
CSB-EP417694CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii DNA-binding protein fis (fis) CSB-YP417695CXL
CSB-EP417695CXL
CSB-BP417695CXL
CSB-MP417695CXL
CSB-EP417695CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii GTPase obg (obg) CSB-YP417696CXL
CSB-EP417696CXL
CSB-BP417696CXL
CSB-MP417696CXL
CSB-EP417696CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii DNA gyrase inhibitor (sbmC) CSB-YP417697CXL
CSB-EP417697CXL
CSB-BP417697CXL
CSB-MP417697CXL
CSB-EP417697CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Putative Holliday junction resolvase (ESA_00393) CSB-YP417698CXL
CSB-EP417698CXL
CSB-BP417698CXL
CSB-MP417698CXL
CSB-EP417698CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP417699CXL
CSB-EP417699CXL
CSB-BP417699CXL
CSB-MP417699CXL
CSB-EP417699CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Glutamyl-tRNA reductase (hemA) CSB-YP417700CXL
CSB-EP417700CXL
CSB-BP417700CXL
CSB-MP417700CXL
CSB-EP417700CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0181 protein ESA_01442 (ESA_01442) CSB-YP417701CXL
CSB-EP417701CXL
CSB-BP417701CXL
CSB-MP417701CXL
CSB-EP417701CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Protein TusC (tusC) CSB-YP417702CXL
CSB-EP417702CXL
CSB-BP417702CXL
CSB-MP417702CXL
CSB-EP417702CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP417703CXL
CSB-EP417703CXL
CSB-BP417703CXL
CSB-MP417703CXL
CSB-EP417703CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP417704CXL
CSB-EP417704CXL
CSB-BP417704CXL
CSB-MP417704CXL
CSB-EP417704CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii L-rhamnose-proton symporter (rhaT), partial CSB-YP417705CXL
CSB-EP417705CXL
CSB-BP417705CXL
CSB-MP417705CXL
CSB-EP417705CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Glucans biosynthesis protein D (mdoD), partial CSB-YP417706CXL
CSB-EP417706CXL
CSB-BP417706CXL
CSB-MP417706CXL
CSB-EP417706CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 50S ribosomal protein L25 (rplY) CSB-YP417707CXL
CSB-EP417707CXL
CSB-BP417707CXL
CSB-MP417707CXL
CSB-EP417707CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Probable Fe (2+)-trafficking protein CSB-YP417708CXL
CSB-EP417708CXL
CSB-BP417708CXL
CSB-MP417708CXL
CSB-EP417708CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 30S ribosomal protein S6 (rpsF) CSB-YP417709CXL
CSB-EP417709CXL
CSB-BP417709CXL
CSB-MP417709CXL
CSB-EP417709CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Thiol:disulfide interchange protein DsbD (dsbD), partial CSB-YP417710CXL1
CSB-EP417710CXL1
CSB-BP417710CXL1
CSB-MP417710CXL1
CSB-EP417710CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0259 membrane protein ESA_01554 (ESA_01554), partial CSB-YP417711CXL1
CSB-EP417711CXL1
CSB-BP417711CXL1
CSB-MP417711CXL1
CSB-EP417711CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Electron transport complex protein RnfG (rnfG), partial CSB-YP417712CXL1
CSB-EP417712CXL1
CSB-BP417712CXL1
CSB-MP417712CXL1
CSB-EP417712CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Low affinity potassium transport system protein kup (kup), partial CSB-YP417713CXL
CSB-EP417713CXL
CSB-BP417713CXL
CSB-MP417713CXL
CSB-EP417713CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>