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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Neurospora crassa Probable Xaa-Pro aminopeptidase NCU11288 (NCU11288) CSB-YP417894NHA
CSB-EP417894NHA
CSB-BP417894NHA
CSB-MP417894NHA
CSB-EP417894NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops flavoviridis Small serum protein 3 CSB-YP417895EZB
CSB-EP417895EZB
CSB-BP417895EZB
CSB-MP417895EZB
CSB-EP417895EZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana palustris Ranatuerin-2PLa CSB-YP417896RJM
CSB-EP417896RJM
CSB-BP417896RJM
CSB-MP417896RJM
CSB-EP417896RJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-ribose pyranase (rbsD) CSB-YP417897SUK
CSB-EP417897SUK
CSB-BP417897SUK
CSB-MP417897SUK
CSB-EP417897SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S18 (rpsR) CSB-YP417898SUK
CSB-EP417898SUK
CSB-BP417898SUK
CSB-MP417898SUK
CSB-EP417898SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lysine--tRNA ligase (lysS) CSB-YP417899SUK
CSB-EP417899SUK
CSB-BP417899SUK
CSB-MP417899SUK
CSB-EP417899SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L11 (rplK) CSB-YP417900SUK
CSB-EP417900SUK
CSB-BP417900SUK
CSB-MP417900SUK
CSB-EP417900SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0741 protein SAHV_0591 (SAHV_0591) CSB-YP417901SUK
CSB-EP417901SUK
CSB-BP417901SUK
CSB-MP417901SUK
CSB-EP417901SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhD2 (mnhD2), partial CSB-YP417902SUK
CSB-EP417902SUK
CSB-BP417902SUK
CSB-MP417902SUK
CSB-EP417902SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0178 protein SAHV_0678 (SAHV_0678) CSB-YP417903SUK
CSB-EP417903SUK
CSB-BP417903SUK
CSB-MP417903SUK
CSB-EP417903SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP417904SUK
CSB-EP417904SUK
CSB-BP417904SUK
CSB-MP417904SUK
CSB-EP417904SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HPr kinase/phosphorylase (hprK) CSB-YP417905SUK
CSB-EP417905SUK
CSB-BP417905SUK
CSB-MP417905SUK
CSB-EP417905SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit F1, partial CSB-YP417906SUK1
CSB-EP417906SUK1
CSB-BP417906SUK1
CSB-MP417906SUK1
CSB-EP417906SUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0344 protein SAHV_0964 (SAHV_0964), partial CSB-YP417907SUK1
CSB-EP417907SUK1
CSB-BP417907SUK1
CSB-MP417907SUK1
CSB-EP417907SUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0738 protein SAHV_0999 (SAHV_0999) CSB-YP417908SUK
CSB-EP417908SUK
CSB-BP417908SUK
CSB-MP417908SUK
CSB-EP417908SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Bifunctional autolysin (atl), partial CSB-YP417909SUK
CSB-EP417909SUK
CSB-BP417909SUK
CSB-MP417909SUK
CSB-EP417909SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0637 protein SAHV_1098 (SAHV_1098) CSB-YP417910SUK
CSB-EP417910SUK
CSB-BP417910SUK
CSB-MP417910SUK
CSB-EP417910SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0348 protein SAHV_1117 (SAHV_1117) CSB-YP417911SUK
CSB-EP417911SUK
CSB-BP417911SUK
CSB-MP417911SUK
CSB-EP417911SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus High-affinity heme uptake system protein isdE (isdE) CSB-YP417912SUK
CSB-EP417912SUK
CSB-BP417912SUK
CSB-MP417912SUK
CSB-EP417912SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus MutS2 protein (mutS2), partial CSB-YP417913SUK
CSB-EP417913SUK
CSB-BP417913SUK
CSB-MP417913SUK
CSB-EP417913SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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