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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Campylobacter hominis ATP-dependent protease subunit HslV (hslV) CSB-YP418294CWD
CSB-EP418294CWD
CSB-BP418294CWD
CSB-MP418294CWD
CSB-EP418294CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Acetate kinase (ackA) CSB-YP418295CWD
CSB-EP418295CWD
CSB-BP418295CWD
CSB-MP418295CWD
CSB-EP418295CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP418296CWD
CSB-EP418296CWD
CSB-BP418296CWD
CSB-MP418296CWD
CSB-EP418296CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Glutamate--tRNA ligase 2 (gltX2) CSB-YP418297CWD
CSB-EP418297CWD
CSB-BP418297CWD
CSB-MP418297CWD
CSB-EP418297CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Deoxycytidine triphosphate deaminase (dcd) CSB-YP418298CWD
CSB-EP418298CWD
CSB-BP418298CWD
CSB-MP418298CWD
CSB-EP418298CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis 50S ribosomal protein L10 (rplJ) CSB-YP418299CWD
CSB-EP418299CWD
CSB-BP418299CWD
CSB-MP418299CWD
CSB-EP418299CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP418300CWD
CSB-EP418300CWD
CSB-BP418300CWD
CSB-MP418300CWD
CSB-EP418300CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Periplasmic nitrate reductase (napA), partial CSB-YP418301CWD
CSB-EP418301CWD
CSB-BP418301CWD
CSB-MP418301CWD
CSB-EP418301CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Protein RecA (recA) CSB-YP418302CWD
CSB-EP418302CWD
CSB-BP418302CWD
CSB-MP418302CWD
CSB-EP418302CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Acetylglutamate kinase (argB) CSB-YP418303MNV
CSB-EP418303MNV
CSB-BP418303MNV
CSB-MP418303MNV
CSB-EP418303MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Tryptophan synthase alpha chain (trpA) CSB-YP418304MNV
CSB-EP418304MNV
CSB-BP418304MNV
CSB-MP418304MNV
CSB-EP418304MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei 30S ribosomal protein S7P (rps7p) CSB-YP418305MNV
CSB-EP418305MNV
CSB-BP418305MNV
CSB-MP418305MNV
CSB-EP418305MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Translation initiation factor 2 subunit beta (eIF2B) CSB-YP418306MNV
CSB-EP418306MNV
CSB-BP418306MNV
CSB-MP418306MNV
CSB-EP418306MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei 30S ribosomal protein S19P (rps19p) CSB-YP418307MNV
CSB-EP418307MNV
CSB-BP418307MNV
CSB-MP418307MNV
CSB-EP418307MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei 30S ribosomal protein S8P (rps8p) CSB-YP418308MNV
CSB-EP418308MNV
CSB-BP418308MNV
CSB-MP418308MNV
CSB-EP418308MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP418309MNV
CSB-EP418309MNV
CSB-BP418309MNV
CSB-MP418309MNV
CSB-EP418309MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Bifunctional enzyme fae/hps (fae-hps) CSB-YP418310MNV
CSB-EP418310MNV
CSB-BP418310MNV
CSB-MP418310MNV
CSB-EP418310MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP418311MNV
CSB-EP418311MNV
CSB-BP418311MNV
CSB-MP418311MNV
CSB-EP418311MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Catalase-peroxidase (katG), partial CSB-YP418312MNV
CSB-EP418312MNV
CSB-BP418312MNV
CSB-MP418312MNV
CSB-EP418312MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei 7-cyano-7-deazaguanine synthase (queC) CSB-YP418313MNV
CSB-EP418313MNV
CSB-BP418313MNV
CSB-MP418313MNV
CSB-EP418313MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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