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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vanderwaltozyma polyspora Protein TIF31 homolog (TIF31), partial CSB-YP418594VDW
CSB-EP418594VDW
CSB-BP418594VDW
CSB-MP418594VDW
CSB-EP418594VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora GDP-mannose transporter 1 (VRG4-1), partial CSB-YP418595VDW
CSB-EP418595VDW
CSB-BP418595VDW
CSB-MP418595VDW
CSB-EP418595VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Histone chaperone RTT106 (RTT106) CSB-YP418596VDW
CSB-EP418596VDW
CSB-BP418596VDW
CSB-MP418596VDW
CSB-EP418596VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora ATP-dependent rRNA helicase RRP3 (RRP3), partial CSB-YP418597VDW
CSB-EP418597VDW
CSB-BP418597VDW
CSB-MP418597VDW
CSB-EP418597VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Altered inheritance of mitochondria protein 21 (AIM21), partial CSB-YP418598VDW
CSB-EP418598VDW
CSB-BP418598VDW
CSB-MP418598VDW
CSB-EP418598VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Vacuolar protein sorting/targeting protein 10 (VPS10), partial CSB-YP418599VDW
CSB-EP418599VDW
CSB-BP418599VDW
CSB-MP418599VDW
CSB-EP418599VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Selection and upkeep of intraepithelial T-cells protein 4 (Skint4), partial CSB-YP418600MO1
CSB-EP418600MO1
CSB-BP418600MO1
CSB-MP418600MO1
CSB-EP418600MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeocystis pouchetii virus Major capsid protein (MCP), partial CSB-YP418601PYK
CSB-EP418601PYK
CSB-BP418601PYK
CSB-MP418601PYK
CSB-EP418601PYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichophyton equinum Subtilisin-like protease 4 (SUB4) CSB-YP418602TPZ
CSB-EP418602TPZ
CSB-BP418602TPZ
CSB-MP418602TPZ
CSB-EP418602TPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa ASTRA-associated protein 1 (asa-1) CSB-YP418603NHA
CSB-EP418603NHA
CSB-BP418603NHA
CSB-MP418603NHA
CSB-EP418603NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops flavoviridis Small serum protein 1 CSB-YP418604EZB
CSB-EP418604EZB
CSB-BP418604EZB
CSB-MP418604EZB
CSB-EP418604EZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L9 (rplI) CSB-YP418605SUK
CSB-EP418605SUK
CSB-BP418605SUK
CSB-MP418605SUK
CSB-EP418605SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable siderophore biosynthesis protein SbnA (sbnA) CSB-YP418606SUK
CSB-EP418606SUK
CSB-BP418606SUK
CSB-MP418606SUK
CSB-EP418606SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus PTS system glucose-specific EIICBA component (ptsG), partial CSB-YP418607SUK
CSB-EP418607SUK
CSB-BP418607SUK
CSB-MP418607SUK
CSB-EP418607SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP418608SUK
CSB-EP418608SUK
CSB-BP418608SUK
CSB-MP418608SUK
CSB-EP418608SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP418609SUK
CSB-EP418609SUK
CSB-BP418609SUK
CSB-MP418609SUK
CSB-EP418609SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L10 (rplJ) CSB-YP418610SUK
CSB-EP418610SUK
CSB-BP418610SUK
CSB-MP418610SUK
CSB-EP418610SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S7 (rpsG) CSB-YP418611SUK
CSB-EP418611SUK
CSB-BP418611SUK
CSB-MP418611SUK
CSB-EP418611SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glucosamine-6-phosphate deaminase (nagB) CSB-YP418612SUK
CSB-EP418612SUK
CSB-BP418612SUK
CSB-MP418612SUK
CSB-EP418612SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhA2 (mnhA2), partial CSB-YP418613SUK
CSB-EP418613SUK
CSB-BP418613SUK
CSB-MP418613SUK
CSB-EP418613SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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