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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus amyloliquefaciens D-alanine--D-alanine ligase (ddl) CSB-YP419394BQD
CSB-EP419394BQD
CSB-BP419394BQD
CSB-MP419394BQD
CSB-EP419394BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP419395BQD
CSB-EP419395BQD
CSB-BP419395BQD
CSB-MP419395BQD
CSB-EP419395BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 3-dehydroquinate dehydratase (aroD) CSB-YP419396BQD
CSB-EP419396BQD
CSB-BP419396BQD
CSB-MP419396BQD
CSB-EP419396BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 30S ribosomal protein S14 (rpsN) CSB-YP419397BQD
CSB-EP419397BQD
CSB-BP419397BQD
CSB-MP419397BQD
CSB-EP419397BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP419398BQD
CSB-EP419398BQD
CSB-BP419398BQD
CSB-MP419398BQD
CSB-EP419398BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UPF0736 protein RBAM_011410 (RBAM_011410) CSB-YP419399BQD
CSB-EP419399BQD
CSB-BP419399BQD
CSB-MP419399BQD
CSB-EP419399BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 7-cyano-7-deazaguanine synthase (queC) CSB-YP419400BQD
CSB-EP419400BQD
CSB-BP419400BQD
CSB-MP419400BQD
CSB-EP419400BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Protoheme IX farnesyltransferase 2 (ctaB2), partial CSB-YP419401BQD1
CSB-EP419401BQD1
CSB-BP419401BQD1
CSB-MP419401BQD1
CSB-EP419401BQD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP419402BQD
CSB-EP419402BQD
CSB-BP419402BQD
CSB-MP419402BQD
CSB-EP419402BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Aspartate carbamoyltransferase (pyrB) CSB-YP419403BQD
CSB-EP419403BQD
CSB-BP419403BQD
CSB-MP419403BQD
CSB-EP419403BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 50S ribosomal protein L19 (rplS) CSB-YP419404BQD
CSB-EP419404BQD
CSB-BP419404BQD
CSB-MP419404BQD
CSB-EP419404BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Ribosome-binding factor A (rbfA) CSB-YP419405BQD
CSB-EP419405BQD
CSB-BP419405BQD
CSB-MP419405BQD
CSB-EP419405BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Ribonuclease Y (rny), partial CSB-YP419406BQD
CSB-EP419406BQD
CSB-BP419406BQD
CSB-MP419406BQD
CSB-EP419406BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Polyketide biosynthesis malonyl CoA-acyl carrier protein transacylase BaeC (baeC) CSB-YP419407BQD
CSB-EP419407BQD
CSB-BP419407BQD
CSB-MP419407BQD
CSB-EP419407BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Cell division suppressor protein YneA (yneA) CSB-YP419408BQD
CSB-EP419408BQD
CSB-BP419408BQD
CSB-MP419408BQD
CSB-EP419408BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Biotin synthase (bioB) CSB-YP419409BQD
CSB-EP419409BQD
CSB-BP419409BQD
CSB-MP419409BQD
CSB-EP419409BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP419410BQD
CSB-EP419410BQD
CSB-BP419410BQD
CSB-MP419410BQD
CSB-EP419410BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UPF0398 protein RBAM_020340 (RBAM_020340) CSB-YP419411BQD
CSB-EP419411BQD
CSB-BP419411BQD
CSB-MP419411BQD
CSB-EP419411BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Methylglyoxal synthase (mgsA) CSB-YP419412BQD
CSB-EP419412BQD
CSB-BP419412BQD
CSB-MP419412BQD
CSB-EP419412BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Indole-3-glycerol phosphate synthase (trpC) CSB-YP419413BQD
CSB-EP419413BQD
CSB-BP419413BQD
CSB-MP419413BQD
CSB-EP419413BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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