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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Large-conductance mechanosensitive channel (mscL), partial CSB-YP418634SUK1
CSB-EP418634SUK1
CSB-BP418634SUK1
CSB-MP418634SUK1
CSB-EP418634SUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tryptophan synthase beta chain (trpB) CSB-YP418635SUK
CSB-EP418635SUK
CSB-BP418635SUK
CSB-MP418635SUK
CSB-EP418635SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP418636SUK
CSB-EP418636SUK
CSB-BP418636SUK
CSB-MP418636SUK
CSB-EP418636SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Extracellular matrix-binding protein ebhA (ebhA), partial CSB-YP418637SUK
CSB-EP418637SUK
CSB-BP418637SUK
CSB-MP418637SUK
CSB-EP418637SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus CCA-adding enzyme (cca) CSB-YP418638SUK
CSB-EP418638SUK
CSB-BP418638SUK
CSB-MP418638SUK
CSB-EP418638SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP418639SUK
CSB-EP418639SUK
CSB-BP418639SUK
CSB-MP418639SUK
CSB-EP418639SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP418640SUK
CSB-EP418640SUK
CSB-BP418640SUK
CSB-MP418640SUK
CSB-EP418640SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable rRNA maturation factor (SAHV_1557) CSB-YP418641SUK
CSB-EP418641SUK
CSB-BP418641SUK
CSB-MP418641SUK
CSB-EP418641SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chaperone protein DnaK (dnaK), partial CSB-YP418642SUK
CSB-EP418642SUK
CSB-BP418642SUK
CSB-MP418642SUK
CSB-EP418642SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP418643SUK
CSB-EP418643SUK
CSB-BP418643SUK
CSB-MP418643SUK
CSB-EP418643SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0297 protein SAHV_1604 (SAHV_1604) CSB-YP418644SUK
CSB-EP418644SUK
CSB-BP418644SUK
CSB-MP418644SUK
CSB-EP418644SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L21 (rplU) CSB-YP418645SUK
CSB-EP418645SUK
CSB-BP418645SUK
CSB-MP418645SUK
CSB-EP418645SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP418646SUK
CSB-EP418646SUK
CSB-BP418646SUK
CSB-MP418646SUK
CSB-EP418646SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP418647SUK
CSB-EP418647SUK
CSB-BP418647SUK
CSB-MP418647SUK
CSB-EP418647SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S4 (rpsD) CSB-YP418648SUK
CSB-EP418648SUK
CSB-BP418648SUK
CSB-MP418648SUK
CSB-EP418648SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Leucine--tRNA ligase (leuS), partial CSB-YP418649SUK
CSB-EP418649SUK
CSB-BP418649SUK
CSB-MP418649SUK
CSB-EP418649SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP418650SUK
CSB-EP418650SUK
CSB-BP418650SUK
CSB-MP418650SUK
CSB-EP418650SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trimorphodon biscutatus Toxin 3FTx-Tri2 CSB-YP418651TKQ
CSB-EP418651TKQ
CSB-BP418651TKQ
CSB-MP418651TKQ
CSB-EP418651TKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Foldase protein prsA (prsA) CSB-YP418652SUK
CSB-EP418652SUK
CSB-BP418652SUK
CSB-MP418652SUK
CSB-EP418652SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enhydris polylepis Lectoxin-Enh2 CSB-YP418653EIY
CSB-EP418653EIY
CSB-BP418653EIY
CSB-MP418653EIY
CSB-EP418653EIY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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