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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Anaeromyxobacter sp. NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP418914AUT1
CSB-EP418914AUT1
CSB-BP418914AUT1
CSB-MP418914AUT1
CSB-EP418914AUT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Probable Fe (2+)-trafficking protein CSB-YP418915AUT
CSB-EP418915AUT
CSB-BP418915AUT
CSB-MP418915AUT
CSB-EP418915AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Ribonuclease H (rnhA) CSB-YP418916AUT
CSB-EP418916AUT
CSB-BP418916AUT
CSB-MP418916AUT
CSB-EP418916AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L23 (rplW) CSB-YP418917AUT
CSB-EP418917AUT
CSB-BP418917AUT
CSB-MP418917AUT
CSB-EP418917AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L14 (rplN) CSB-YP418918AUT
CSB-EP418918AUT
CSB-BP418918AUT
CSB-MP418918AUT
CSB-EP418918AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L18 (rplR) CSB-YP418919AUT
CSB-EP418919AUT
CSB-BP418919AUT
CSB-MP418919AUT
CSB-EP418919AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 30S ribosomal protein S13 (rpsM) CSB-YP418920AUT
CSB-EP418920AUT
CSB-BP418920AUT
CSB-MP418920AUT
CSB-EP418920AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. tRNA (guanine-N (1)-)-methyltransferase CSB-YP418921AUT
CSB-EP418921AUT
CSB-BP418921AUT
CSB-MP418921AUT
CSB-EP418921AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. DNA mismatch repair protein mutL (mutL), partial CSB-YP418922AUT
CSB-EP418922AUT
CSB-BP418922AUT
CSB-MP418922AUT
CSB-EP418922AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L1 (rplA) CSB-YP418923AUT
CSB-EP418923AUT
CSB-BP418923AUT
CSB-MP418923AUT
CSB-EP418923AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Globin-coupled histidine kinase (gchK) CSB-YP418924AUT
CSB-EP418924AUT
CSB-BP418924AUT
CSB-MP418924AUT
CSB-EP418924AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Bifunctional enzyme IspD/IspF (ispDF) CSB-YP418925AUT
CSB-EP418925AUT
CSB-BP418925AUT
CSB-MP418925AUT
CSB-EP418925AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Putative Holliday junction resolvase (Anae109_2535) CSB-YP418926AUT
CSB-EP418926AUT
CSB-BP418926AUT
CSB-MP418926AUT
CSB-EP418926AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP418927AUT
CSB-EP418927AUT
CSB-BP418927AUT
CSB-MP418927AUT
CSB-EP418927AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 8-amino-7-oxononanoate synthase (bioF) CSB-YP418928AUT
CSB-EP418928AUT
CSB-BP418928AUT
CSB-MP418928AUT
CSB-EP418928AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. SsrA-binding protein (smpB) CSB-YP418929AUT
CSB-EP418929AUT
CSB-BP418929AUT
CSB-MP418929AUT
CSB-EP418929AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP418930AUT
CSB-EP418930AUT
CSB-BP418930AUT
CSB-MP418930AUT
CSB-EP418930AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Protein-L-isoaspartate O-methyltransferase 2 (pcm2) CSB-YP418931AUT
CSB-EP418931AUT
CSB-BP418931AUT
CSB-MP418931AUT
CSB-EP418931AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. GTPase obg (obg) CSB-YP418932AUT
CSB-EP418932AUT
CSB-BP418932AUT
CSB-MP418932AUT
CSB-EP418932AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Putative 3-methyladenine DNA glycosylase (Anae109_4380) CSB-YP418933AUT
CSB-EP418933AUT
CSB-BP418933AUT
CSB-MP418933AUT
CSB-EP418933AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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