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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O9:H4 Met repressor (metJ) CSB-YP420055EJF
CSB-EP420055EJF
CSB-BP420055EJF
CSB-MP420055EJF
CSB-EP420055EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 HTH-type transcriptional repressor fabR (fabR) CSB-YP420056EJF
CSB-EP420056EJF
CSB-BP420056EJF
CSB-MP420056EJF
CSB-EP420056EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 50S ribosomal protein L10 (rplJ) CSB-YP420057EJF
CSB-EP420057EJF
CSB-BP420057EJF
CSB-MP420057EJF
CSB-EP420057EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 NADH pyrophosphatase (nudC) CSB-YP420058EJF
CSB-EP420058EJF
CSB-BP420058EJF
CSB-MP420058EJF
CSB-EP420058EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Peptidase E (pepE) CSB-YP420059EJF
CSB-EP420059EJF
CSB-BP420059EJF
CSB-MP420059EJF
CSB-EP420059EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 LexA repressor (lexA) CSB-YP420060EJF
CSB-EP420060EJF
CSB-BP420060EJF
CSB-MP420060EJF
CSB-EP420060EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Fumarate reductase subunit C (frdC), partial CSB-YP420061EJF1
CSB-EP420061EJF1
CSB-BP420061EJF1
CSB-MP420061EJF1
CSB-EP420061EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 HTH-type transcriptional regulator UlaR (ulaR) CSB-YP420062EJF
CSB-EP420062EJF
CSB-BP420062EJF
CSB-MP420062EJF
CSB-EP420062EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP420063EJF
CSB-EP420063EJF
CSB-BP420063EJF
CSB-MP420063EJF
CSB-EP420063EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Primosomal protein 1 (dnaT) CSB-YP420064EJF
CSB-EP420064EJF
CSB-BP420064EJF
CSB-MP420064EJF
CSB-EP420064EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Lipoate-protein ligase A (lplA) CSB-YP420065EJF
CSB-EP420065EJF
CSB-BP420065EJF
CSB-MP420065EJF
CSB-EP420065EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis UPF0218 protein Igni_0082 (Igni_0082) CSB-YP420066ILH
CSB-EP420066ILH
CSB-BP420066ILH
CSB-MP420066ILH
CSB-EP420066ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis DNA/RNA-binding protein Alba (albA) CSB-YP420067ILH
CSB-EP420067ILH
CSB-BP420067ILH
CSB-MP420067ILH
CSB-EP420067ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis 50S ribosomal protein L7Ae (rpl7Ae) CSB-YP420068ILH
CSB-EP420068ILH
CSB-BP420068ILH
CSB-MP420068ILH
CSB-EP420068ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis 50S ribosomal protein L31e (rpl31e) CSB-YP420069ILH
CSB-EP420069ILH
CSB-BP420069ILH
CSB-MP420069ILH
CSB-EP420069ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Elongation factor 1-beta (ef1b) CSB-YP420070ILH
CSB-EP420070ILH
CSB-BP420070ILH
CSB-MP420070ILH
CSB-EP420070ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Ferredoxin--NADP reductase (Igni_0617) CSB-YP420071ILH
CSB-EP420071ILH
CSB-BP420071ILH
CSB-MP420071ILH
CSB-EP420071ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Arginine decarboxylase proenzyme (Igni_0686) CSB-YP420072ILH
CSB-EP420072ILH
CSB-BP420072ILH
CSB-MP420072ILH
CSB-EP420072ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP420073ILH
CSB-EP420073ILH
CSB-BP420073ILH
CSB-MP420073ILH
CSB-EP420073ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis 50S ribosomal protein L40e (rpl40e) CSB-YP420074ILH
CSB-EP420074ILH
CSB-BP420074ILH
CSB-MP420074ILH
CSB-EP420074ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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