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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Citrobacter koseri Xanthine phosphoribosyltransferase (gpt) CSB-YP420115CWS
CSB-EP420115CWS
CSB-BP420115CWS
CSB-MP420115CWS
CSB-EP420115CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Phosphomethylpyrimidine synthase (thiC), partial CSB-YP420116CWS
CSB-EP420116CWS
CSB-BP420116CWS
CSB-MP420116CWS
CSB-EP420116CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 50S ribosomal protein L11 (rplK) CSB-YP420117CWS
CSB-EP420117CWS
CSB-BP420117CWS
CSB-MP420117CWS
CSB-EP420117CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Catalase-peroxidase (katG), partial CSB-YP420118CWS
CSB-EP420118CWS
CSB-BP420118CWS
CSB-MP420118CWS
CSB-EP420118CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri D-tyrosyl-tRNA (Tyr) deacylase CSB-YP420119CWS
CSB-EP420119CWS
CSB-BP420119CWS
CSB-MP420119CWS
CSB-EP420119CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP420120CWS
CSB-EP420120CWS
CSB-BP420120CWS
CSB-MP420120CWS
CSB-EP420120CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP420121CWS
CSB-EP420121CWS
CSB-BP420121CWS
CSB-MP420121CWS
CSB-EP420121CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri UPF0231 protein CKO_03249 (CKO_03249) CSB-YP420122CWS
CSB-EP420122CWS
CSB-BP420122CWS
CSB-MP420122CWS
CSB-EP420122CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP420123CWS
CSB-EP420123CWS
CSB-BP420123CWS
CSB-MP420123CWS
CSB-EP420123CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri L-arabinose isomerase (araA) CSB-YP420124CWS
CSB-EP420124CWS
CSB-BP420124CWS
CSB-MP420124CWS
CSB-EP420124CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Isoleucine--tRNA ligase (ileS), partial CSB-YP420125CWS
CSB-EP420125CWS
CSB-BP420125CWS
CSB-MP420125CWS
CSB-EP420125CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP420126CWS
CSB-EP420126CWS
CSB-BP420126CWS
CSB-MP420126CWS
CSB-EP420126CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Probable cytosol aminopeptidase (pepA), partial CSB-YP420127CWS
CSB-EP420127CWS
CSB-BP420127CWS
CSB-MP420127CWS
CSB-EP420127CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Primosomal replication protein n (priB) CSB-YP420128CWS
CSB-EP420128CWS
CSB-BP420128CWS
CSB-MP420128CWS
CSB-EP420128CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Uncharacterized protein YjeA (yjeA) CSB-YP420129CWS
CSB-EP420129CWS
CSB-BP420129CWS
CSB-MP420129CWS
CSB-EP420129CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Homoserine O-succinyltransferase (metA) CSB-YP420130CWS
CSB-EP420130CWS
CSB-BP420130CWS
CSB-MP420130CWS
CSB-EP420130CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri S-ribosylhomocysteine lyase (luxS) CSB-YP420131CWS
CSB-EP420131CWS
CSB-BP420131CWS
CSB-MP420131CWS
CSB-EP420131CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Nitric oxide reductase FlRd-NAD (+) reductase CSB-YP420132CWS
CSB-EP420132CWS
CSB-BP420132CWS
CSB-MP420132CWS
CSB-EP420132CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP420133CWS
CSB-EP420133CWS
CSB-BP420133CWS
CSB-MP420133CWS
CSB-EP420133CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Protein syd (syd) CSB-YP420134CWS
CSB-EP420134CWS
CSB-BP420134CWS
CSB-MP420134CWS
CSB-EP420134CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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