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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Arginine--tRNA ligase (argS), partial CSB-YP420376CWG
CSB-EP420376CWG
CSB-BP420376CWG
CSB-MP420376CWG
CSB-EP420376CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP420377CWG
CSB-EP420377CWG
CSB-BP420377CWG
CSB-MP420377CWG
CSB-EP420377CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP420378CWG
CSB-EP420378CWG
CSB-BP420378CWG
CSB-MP420378CWG
CSB-EP420378CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP420379CWG
CSB-EP420379CWG
CSB-BP420379CWG
CSB-MP420379CWG
CSB-EP420379CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Glutamate--tRNA ligase 2 (gltX2) CSB-YP420380CWG
CSB-EP420380CWG
CSB-BP420380CWG
CSB-MP420380CWG
CSB-EP420380CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Polyphosphate kinase (ppk), partial CSB-YP420381CWG
CSB-EP420381CWG
CSB-BP420381CWG
CSB-MP420381CWG
CSB-EP420381CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 UPF0306 protein C8J_1355 (C8J_1355) CSB-YP420382CWG
CSB-EP420382CWG
CSB-BP420382CWG
CSB-MP420382CWG
CSB-EP420382CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Diaminopimelate epimerase (dapF) CSB-YP420383CWG
CSB-EP420383CWG
CSB-BP420383CWG
CSB-MP420383CWG
CSB-EP420383CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Translation initiation factor IF-1 (infA) CSB-YP420384CWG
CSB-EP420384CWG
CSB-BP420384CWG
CSB-MP420384CWG
CSB-EP420384CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 50S ribosomal protein L17 (rplQ) CSB-YP420385CWG
CSB-EP420385CWG
CSB-BP420385CWG
CSB-MP420385CWG
CSB-EP420385CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Bifunctional enzyme IspD/IspF (ispDF) CSB-YP420386CWG
CSB-EP420386CWG
CSB-BP420386CWG
CSB-MP420386CWG
CSB-EP420386CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Protein RecA (recA) CSB-YP420387CWG
CSB-EP420387CWG
CSB-BP420387CWG
CSB-MP420387CWG
CSB-EP420387CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 50S ribosomal protein L24 (rplX) CSB-YP420388CWG
CSB-EP420388CWG
CSB-BP420388CWG
CSB-MP420388CWG
CSB-EP420388CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 50S ribosomal protein L23 (rplW) CSB-YP420389CWG
CSB-EP420389CWG
CSB-BP420389CWG
CSB-MP420389CWG
CSB-EP420389CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP420390CWG
CSB-EP420390CWG
CSB-BP420390CWG
CSB-MP420390CWG
CSB-EP420390CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP420391STR
CSB-EP420391STR
CSB-BP420391STR
CSB-MP420391STR
CSB-EP420391STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP420392STR
CSB-EP420392STR
CSB-BP420392STR
CSB-MP420392STR
CSB-EP420392STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Phosphopantetheine adenylyltransferase (coaD) CSB-YP420393STR
CSB-EP420393STR
CSB-BP420393STR
CSB-MP420393STR
CSB-EP420393STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis D-tyrosyl-tRNA (Tyr) deacylase CSB-YP420394STR
CSB-EP420394STR
CSB-BP420394STR
CSB-MP420394STR
CSB-EP420394STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Nucleoid occlusion factor SlmA (slmA) CSB-YP420395STR
CSB-EP420395STR
CSB-BP420395STR
CSB-MP420395STR
CSB-EP420395STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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