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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Streptococcus gordonii DNA repair protein recO (recO) CSB-YP421176FMN
CSB-EP421176FMN
CSB-BP421176FMN
CSB-MP421176FMN
CSB-EP421176FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP421177FMN
CSB-EP421177FMN
CSB-BP421177FMN
CSB-MP421177FMN
CSB-EP421177FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Phosphoglycerate kinase (pgk) CSB-YP421178FMN
CSB-EP421178FMN
CSB-BP421178FMN
CSB-MP421178FMN
CSB-EP421178FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii DNA polymerase IV (dinB) CSB-YP421179FMN
CSB-EP421179FMN
CSB-BP421179FMN
CSB-MP421179FMN
CSB-EP421179FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 30S ribosomal protein S15 (rpsO) CSB-YP421180FMN
CSB-EP421180FMN
CSB-BP421180FMN
CSB-MP421180FMN
CSB-EP421180FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Chaperone protein DnaK (dnaK), partial CSB-YP421181FMN
CSB-EP421181FMN
CSB-BP421181FMN
CSB-MP421181FMN
CSB-EP421181FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP421182FMN
CSB-EP421182FMN
CSB-BP421182FMN
CSB-MP421182FMN
CSB-EP421182FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP421183FMN
CSB-EP421183FMN
CSB-BP421183FMN
CSB-MP421183FMN
CSB-EP421183FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Ribosome maturation factor RimP (rimP) CSB-YP421184FMN
CSB-EP421184FMN
CSB-BP421184FMN
CSB-MP421184FMN
CSB-EP421184FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii UPF0291 protein SGO_0570 (SGO_0570) CSB-YP421185FMN
CSB-EP421185FMN
CSB-BP421185FMN
CSB-MP421185FMN
CSB-EP421185FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Phosphopantetheine adenylyltransferase (coaD) CSB-YP421186FMN
CSB-EP421186FMN
CSB-BP421186FMN
CSB-MP421186FMN
CSB-EP421186FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP421187FMN
CSB-EP421187FMN
CSB-BP421187FMN
CSB-MP421187FMN
CSB-EP421187FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP421188FMN1
CSB-EP421188FMN1
CSB-BP421188FMN1
CSB-MP421188FMN1
CSB-EP421188FMN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Fructose-1,6-bisphosphatase class 3 (fbp), partial CSB-YP421189FMN
CSB-EP421189FMN
CSB-BP421189FMN
CSB-MP421189FMN
CSB-EP421189FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP421190FMN
CSB-EP421190FMN
CSB-BP421190FMN
CSB-MP421190FMN
CSB-EP421190FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii S-adenosylmethionine synthase (metK) CSB-YP421191FMN
CSB-EP421191FMN
CSB-BP421191FMN
CSB-MP421191FMN
CSB-EP421191FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii UPF0223 protein SGO_1052 (SGO_1052) CSB-YP421192FMN
CSB-EP421192FMN
CSB-BP421192FMN
CSB-MP421192FMN
CSB-EP421192FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Dihydrodipicolinate reductase (dapB) CSB-YP421193FMN
CSB-EP421193FMN
CSB-BP421193FMN
CSB-MP421193FMN
CSB-EP421193FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Xanthine phosphoribosyltransferase (xpt) CSB-YP421194FMN
CSB-EP421194FMN
CSB-BP421194FMN
CSB-MP421194FMN
CSB-EP421194FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP421195FMN
CSB-EP421195FMN
CSB-BP421195FMN
CSB-MP421195FMN
CSB-EP421195FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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