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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O139:H28 UPF0213 protein yhbQ (yhbQ) CSB-YP421976EJD
CSB-EP421976EJD
CSB-BP421976EJD
CSB-MP421976EJD
CSB-EP421976EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Phosphoglucosamine mutase (glmM) CSB-YP421977EJD
CSB-EP421977EJD
CSB-BP421977EJD
CSB-MP421977EJD
CSB-EP421977EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 N-acetylmannosamine kinase (nanK) CSB-YP421978EJD
CSB-EP421978EJD
CSB-BP421978EJD
CSB-MP421978EJD
CSB-EP421978EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 p-hydroxybenzoic acid efflux pump subunit AaeB (aaeB), partial CSB-YP421979EJD
CSB-EP421979EJD
CSB-BP421979EJD
CSB-MP421979EJD
CSB-EP421979EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP421980EJD
CSB-EP421980EJD
CSB-BP421980EJD
CSB-MP421980EJD
CSB-EP421980EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 50S ribosomal protein L30 (rpmD) CSB-YP421981EJD
CSB-EP421981EJD
CSB-BP421981EJD
CSB-MP421981EJD
CSB-EP421981EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 50S ribosomal protein L14 (rplN) CSB-YP421982EJD
CSB-EP421982EJD
CSB-BP421982EJD
CSB-MP421982EJD
CSB-EP421982EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 50S ribosomal protein L4 (rplD) CSB-YP421983EJD
CSB-EP421983EJD
CSB-BP421983EJD
CSB-MP421983EJD
CSB-EP421983EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Protein TusC (tusC) CSB-YP421984EJD
CSB-EP421984EJD
CSB-BP421984EJD
CSB-MP421984EJD
CSB-EP421984EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Shikimate kinase 1 (aroK) CSB-YP421985EJD
CSB-EP421985EJD
CSB-BP421985EJD
CSB-MP421985EJD
CSB-EP421985EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Glycogen synthase (glgA) CSB-YP421986EJD
CSB-EP421986EJD
CSB-BP421986EJD
CSB-MP421986EJD
CSB-EP421986EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP421987EJD
CSB-EP421987EJD
CSB-BP421987EJD
CSB-MP421987EJD
CSB-EP421987EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 L-seryl-tRNA (Sec) selenium transferase CSB-YP421988EJD
CSB-EP421988EJD
CSB-BP421988EJD
CSB-MP421988EJD
CSB-EP421988EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP421989EJD
CSB-EP421989EJD
CSB-BP421989EJD
CSB-MP421989EJD
CSB-EP421989EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 DNA ligase B (ligB) CSB-YP421990EJD
CSB-EP421990EJD
CSB-BP421990EJD
CSB-MP421990EJD
CSB-EP421990EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Chromosomal replication initiator protein DnaA (dnaA) CSB-YP421991EJD
CSB-EP421991EJD
CSB-BP421991EJD
CSB-MP421991EJD
CSB-EP421991EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Tryptophanase (tnaA) CSB-YP421992EJD
CSB-EP421992EJD
CSB-BP421992EJD
CSB-MP421992EJD
CSB-EP421992EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 ATP synthase epsilon chain (atpC) CSB-YP421993EJD
CSB-EP421993EJD
CSB-BP421993EJD
CSB-MP421993EJD
CSB-EP421993EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) CSB-YP421994EJD
CSB-EP421994EJD
CSB-BP421994EJD
CSB-MP421994EJD
CSB-EP421994EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 D-ribose pyranase (rbsD) CSB-YP421995EJD
CSB-EP421995EJD
CSB-BP421995EJD
CSB-MP421995EJD
CSB-EP421995EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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