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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit N CSB-YP420476PZD
CSB-EP420476PZD
CSB-BP420476PZD
CSB-MP420476PZD
CSB-EP420476PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosome maturation factor RimM (rimM) CSB-YP420477PZD
CSB-EP420477PZD
CSB-BP420477PZD
CSB-MP420477PZD
CSB-EP420477PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP420478PZD
CSB-EP420478PZD
CSB-BP420478PZD
CSB-MP420478PZD
CSB-EP420478PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP420479PZD
CSB-EP420479PZD
CSB-BP420479PZD
CSB-MP420479PZD
CSB-EP420479PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP420480PZD
CSB-EP420480PZD
CSB-BP420480PZD
CSB-MP420480PZD
CSB-EP420480PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Ribonuclease P protein component (rnpA) CSB-YP420481STJ
CSB-EP420481STJ
CSB-BP420481STJ
CSB-MP420481STJ
CSB-EP420481STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Putative transport protein Spro_0050 (Spro_0050) CSB-YP420482STJ
CSB-EP420482STJ
CSB-BP420482STJ
CSB-MP420482STJ
CSB-EP420482STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans C4-dicarboxylate transport protein (dctA), partial CSB-YP420483STJ
CSB-EP420483STJ
CSB-BP420483STJ
CSB-MP420483STJ
CSB-EP420483STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Tat-linked quality control protein TatD (tatD) CSB-YP420484STJ
CSB-EP420484STJ
CSB-BP420484STJ
CSB-MP420484STJ
CSB-EP420484STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L11 (rplK) CSB-YP420485STJ
CSB-EP420485STJ
CSB-BP420485STJ
CSB-MP420485STJ
CSB-EP420485STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Fumarate reductase subunit D (frdD), partial CSB-YP420486STJ1
CSB-EP420486STJ1
CSB-BP420486STJ1
CSB-MP420486STJ1
CSB-EP420486STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein hfq (hfq) CSB-YP420487STJ
CSB-EP420487STJ
CSB-BP420487STJ
CSB-MP420487STJ
CSB-EP420487STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L21 (rplU) CSB-YP420488STJ
CSB-EP420488STJ
CSB-BP420488STJ
CSB-MP420488STJ
CSB-EP420488STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Ribosome-binding factor A (rbfA) CSB-YP420489STJ
CSB-EP420489STJ
CSB-BP420489STJ
CSB-MP420489STJ
CSB-EP420489STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0246 protein Spro_0686 (Spro_0686) CSB-YP420490STJ
CSB-EP420490STJ
CSB-BP420490STJ
CSB-MP420490STJ
CSB-EP420490STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP420491STJ
CSB-EP420491STJ
CSB-BP420491STJ
CSB-MP420491STJ
CSB-EP420491STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans RNA polymerase-associated protein RapA (rapA), partial CSB-YP420492STJ
CSB-EP420492STJ
CSB-BP420492STJ
CSB-MP420492STJ
CSB-EP420492STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP420493STJ
CSB-EP420493STJ
CSB-BP420493STJ
CSB-MP420493STJ
CSB-EP420493STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), partial CSB-YP420494STJ
CSB-EP420494STJ
CSB-BP420494STJ
CSB-MP420494STJ
CSB-EP420494STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Cell division protein FtsB (ftsB), partial CSB-YP420495STJ
CSB-EP420495STJ
CSB-BP420495STJ
CSB-MP420495STJ
CSB-EP420495STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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