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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rickettsia massiliae 50S ribosomal protein L19 (rplS) CSB-YP421276RIN
CSB-EP421276RIN
CSB-BP421276RIN
CSB-MP421276RIN
CSB-EP421276RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae UPF0176 protein RMA_0175 (RMA_0175) CSB-YP421277RIN
CSB-EP421277RIN
CSB-BP421277RIN
CSB-MP421277RIN
CSB-EP421277RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Probable cytosol aminopeptidase (pepA) CSB-YP421278RIN
CSB-EP421278RIN
CSB-BP421278RIN
CSB-MP421278RIN
CSB-EP421278RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae UvrABC system protein B (uvrB), partial CSB-YP421279RIN
CSB-EP421279RIN
CSB-BP421279RIN
CSB-MP421279RIN
CSB-EP421279RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae D-alanine--D-alanine ligase (ddl) CSB-YP421280RIN
CSB-EP421280RIN
CSB-BP421280RIN
CSB-MP421280RIN
CSB-EP421280RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae 30S ribosomal protein S4 (rpsD) CSB-YP421281RIN
CSB-EP421281RIN
CSB-BP421281RIN
CSB-MP421281RIN
CSB-EP421281RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP421282RIN
CSB-EP421282RIN
CSB-BP421282RIN
CSB-MP421282RIN
CSB-EP421282RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Dihydrodipicolinate synthase (dapA) CSB-YP421283RIN
CSB-EP421283RIN
CSB-BP421283RIN
CSB-MP421283RIN
CSB-EP421283RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP421284RIN
CSB-EP421284RIN
CSB-BP421284RIN
CSB-MP421284RIN
CSB-EP421284RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae 50S ribosomal protein L17 (rplQ) CSB-YP421285RIN
CSB-EP421285RIN
CSB-BP421285RIN
CSB-MP421285RIN
CSB-EP421285RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae 30S ribosomal protein S14 (rpsN) CSB-YP421286RIN
CSB-EP421286RIN
CSB-BP421286RIN
CSB-MP421286RIN
CSB-EP421286RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae 30S ribosomal protein S19 (rpsS) CSB-YP421287RIN
CSB-EP421287RIN
CSB-BP421287RIN
CSB-MP421287RIN
CSB-EP421287RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Hemolysin C homolog (tlyC) CSB-YP421288RIN
CSB-EP421288RIN
CSB-BP421288RIN
CSB-MP421288RIN
CSB-EP421288RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP421289RIN
CSB-EP421289RIN
CSB-BP421289RIN
CSB-MP421289RIN
CSB-EP421289RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae 50S ribosomal protein L33 (rpmG) CSB-YP421290RIN
CSB-EP421290RIN
CSB-BP421290RIN
CSB-MP421290RIN
CSB-EP421290RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Chromosomal replication initiator protein DnaA (dnaA) CSB-YP421291TKE
CSB-EP421291TKE
CSB-BP421291TKE
CSB-MP421291TKE
CSB-EP421291TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae 30S ribosomal protein S16 (rpsP) CSB-YP421292TKE
CSB-EP421292TKE
CSB-BP421292TKE
CSB-MP421292TKE
CSB-EP421292TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP421293TKE
CSB-EP421293TKE
CSB-BP421293TKE
CSB-MP421293TKE
CSB-EP421293TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae 10 kDa chaperonin (groS) CSB-YP421294TKE
CSB-EP421294TKE
CSB-BP421294TKE
CSB-MP421294TKE
CSB-EP421294TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae Isoleucine--tRNA ligase (ileS), partial CSB-YP421295TKE
CSB-EP421295TKE
CSB-BP421295TKE
CSB-MP421295TKE
CSB-EP421295TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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