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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Serratia proteamaculans Cytoskeleton protein rodZ (rodZ), partial CSB-YP420536STJ
CSB-EP420536STJ
CSB-BP420536STJ
CSB-MP420536STJ
CSB-EP420536STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans DNA repair protein recO (recO) CSB-YP420537STJ
CSB-EP420537STJ
CSB-BP420537STJ
CSB-MP420537STJ
CSB-EP420537STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP420538STJ
CSB-EP420538STJ
CSB-BP420538STJ
CSB-MP420538STJ
CSB-EP420538STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans tRNA (Ile)-lysidine synthase CSB-YP420539STJ
CSB-EP420539STJ
CSB-BP420539STJ
CSB-MP420539STJ
CSB-EP420539STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0325 protein Spro_3794 (Spro_3794) CSB-YP420540STJ
CSB-EP420540STJ
CSB-BP420540STJ
CSB-MP420540STJ
CSB-EP420540STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans RNA pyrophosphohydrolase (rppH) CSB-YP420541STJ
CSB-EP420541STJ
CSB-BP420541STJ
CSB-MP420541STJ
CSB-EP420541STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Arginine exporter protein ArgO (argO), partial CSB-YP420542STJ1
CSB-EP420542STJ1
CSB-BP420542STJ1
CSB-MP420542STJ1
CSB-EP420542STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Aspartate 1-decarboxylase (panD) CSB-YP420543STJ
CSB-EP420543STJ
CSB-BP420543STJ
CSB-MP420543STJ
CSB-EP420543STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Putative Holliday junction resolvase (Spro_4028) CSB-YP420544STJ
CSB-EP420544STJ
CSB-BP420544STJ
CSB-MP420544STJ
CSB-EP420544STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Bifunctional protein HldE (hldE) CSB-YP420545STJ
CSB-EP420545STJ
CSB-BP420545STJ
CSB-MP420545STJ
CSB-EP420545STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 30S ribosomal protein S21 (rpsU) CSB-YP420546STJ
CSB-EP420546STJ
CSB-BP420546STJ
CSB-MP420546STJ
CSB-EP420546STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Penicillin-binding protein activator LpoA (lpoA), partial CSB-YP420547STJ
CSB-EP420547STJ
CSB-BP420547STJ
CSB-MP420547STJ
CSB-EP420547STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0042 nucleotide-binding protein Spro_4370 (Spro_4370) CSB-YP420548STJ
CSB-EP420548STJ
CSB-BP420548STJ
CSB-MP420548STJ
CSB-EP420548STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans DNA-binding protein fis (fis) CSB-YP420549STJ
CSB-EP420549STJ
CSB-BP420549STJ
CSB-MP420549STJ
CSB-EP420549STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein psiE homolog (psiE), partial CSB-YP420550STJ1
CSB-EP420550STJ1
CSB-BP420550STJ1
CSB-MP420550STJ1
CSB-EP420550STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP420551STJ
CSB-EP420551STJ
CSB-BP420551STJ
CSB-MP420551STJ
CSB-EP420551STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L30 (rpmD) CSB-YP420552STJ
CSB-EP420552STJ
CSB-BP420552STJ
CSB-MP420552STJ
CSB-EP420552STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L18 (rplR) CSB-YP420553STJ
CSB-EP420553STJ
CSB-BP420553STJ
CSB-MP420553STJ
CSB-EP420553STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 30S ribosomal protein S17 (rpsQ) CSB-YP420554STJ
CSB-EP420554STJ
CSB-BP420554STJ
CSB-MP420554STJ
CSB-EP420554STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L2 (rplB) CSB-YP420555STJ
CSB-EP420555STJ
CSB-BP420555STJ
CSB-MP420555STJ
CSB-EP420555STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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