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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O139:H28 UPF0176 protein yceA (yceA) CSB-YP421936EJD
CSB-EP421936EJD
CSB-BP421936EJD
CSB-MP421936EJD
CSB-EP421936EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Phosphate acyltransferase (plsX) CSB-YP421937EJD
CSB-EP421937EJD
CSB-BP421937EJD
CSB-MP421937EJD
CSB-EP421937EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Peptidase T (pepT) CSB-YP421938EJD
CSB-EP421938EJD
CSB-BP421938EJD
CSB-MP421938EJD
CSB-EP421938EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0745 protein YcgL (ycgL) CSB-YP421939EJD
CSB-EP421939EJD
CSB-BP421939EJD
CSB-MP421939EJD
CSB-EP421939EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Outer-membrane lipoprotein lolB (lolB) CSB-YP421940EJD
CSB-EP421940EJD
CSB-BP421940EJD
CSB-MP421940EJD
CSB-EP421940EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP421941EJD
CSB-EP421941EJD
CSB-BP421941EJD
CSB-MP421941EJD
CSB-EP421941EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Probable sugar efflux transporter (sotB), partial CSB-YP421942EJD
CSB-EP421942EJD
CSB-BP421942EJD
CSB-MP421942EJD
CSB-EP421942EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Spermidine export protein MdtJ (mdtJ), partial CSB-YP421943EJD1
CSB-EP421943EJD1
CSB-BP421943EJD1
CSB-MP421943EJD1
CSB-EP421943EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Electron transport complex protein RnfC (rnfC) CSB-YP421944EJD
CSB-EP421944EJD
CSB-BP421944EJD
CSB-MP421944EJD
CSB-EP421944EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Multidrug resistance protein MdtK (mdtK), partial CSB-YP421945EJD
CSB-EP421945EJD
CSB-BP421945EJD
CSB-MP421945EJD
CSB-EP421945EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP421946EJD
CSB-EP421946EJD
CSB-BP421946EJD
CSB-MP421946EJD
CSB-EP421946EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Protein ves (ves) CSB-YP421947EJD
CSB-EP421947EJD
CSB-BP421947EJD
CSB-MP421947EJD
CSB-EP421947EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0229 protein yeaH (yeaH) CSB-YP421948EJD
CSB-EP421948EJD
CSB-BP421948EJD
CSB-MP421948EJD
CSB-EP421948EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP421949EJD
CSB-EP421949EJD
CSB-BP421949EJD
CSB-MP421949EJD
CSB-EP421949EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 tRNA (cmo5U34)-methyltransferase CSB-YP421950EJD
CSB-EP421950EJD
CSB-BP421950EJD
CSB-MP421950EJD
CSB-EP421950EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Flagellar hook-basal body complex protein FliE (fliE) CSB-YP421951EJD
CSB-EP421951EJD
CSB-BP421951EJD
CSB-MP421951EJD
CSB-EP421951EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP421952EJD
CSB-EP421952EJD
CSB-BP421952EJD
CSB-MP421952EJD
CSB-EP421952EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Multidrug resistance protein MdtA (mdtA) CSB-YP421953EJD
CSB-EP421953EJD
CSB-BP421953EJD
CSB-MP421953EJD
CSB-EP421953EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Transcriptional repressor rcnR homolog (rcnR) CSB-YP421954EJD
CSB-EP421954EJD
CSB-BP421954EJD
CSB-MP421954EJD
CSB-EP421954EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Pyrimidine-specific ribonucleoside hydrolase rihB (rihB) CSB-YP421955EJD
CSB-EP421955EJD
CSB-BP421955EJD
CSB-MP421955EJD
CSB-EP421955EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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