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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella sediminis tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP421416STR
CSB-EP421416STR
CSB-BP421416STR
CSB-MP421416STR
CSB-EP421416STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP421417STR
CSB-EP421417STR
CSB-BP421417STR
CSB-MP421417STR
CSB-EP421417STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 50S ribosomal protein L33 (rpmG) CSB-YP421418STR
CSB-EP421418STR
CSB-BP421418STR
CSB-MP421418STR
CSB-EP421418STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP421419STR
CSB-EP421419STR
CSB-BP421419STR
CSB-MP421419STR
CSB-EP421419STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP421420STR
CSB-EP421420STR
CSB-BP421420STR
CSB-MP421420STR
CSB-EP421420STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP421421STR1
CSB-EP421421STR1
CSB-BP421421STR1
CSB-MP421421STR1
CSB-EP421421STR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 10 kDa chaperonin (groS) CSB-YP421422STR
CSB-EP421422STR
CSB-BP421422STR
CSB-MP421422STR
CSB-EP421422STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 30S ribosomal protein S6 (rpsF) CSB-YP421423STR
CSB-EP421423STR
CSB-BP421423STR
CSB-MP421423STR
CSB-EP421423STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 50S ribosomal protein L9 (rplI) CSB-YP421424STR
CSB-EP421424STR
CSB-BP421424STR
CSB-MP421424STR
CSB-EP421424STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis tRNA dimethylallyltransferase 1 (miaA1) CSB-YP421425STR
CSB-EP421425STR
CSB-BP421425STR
CSB-MP421425STR
CSB-EP421425STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP421426STR
CSB-EP421426STR
CSB-BP421426STR
CSB-MP421426STR
CSB-EP421426STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Bifunctional polymyxin resistance protein ArnA (arnA), partial CSB-YP421427STR
CSB-EP421427STR
CSB-BP421427STR
CSB-MP421427STR
CSB-EP421427STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 50S ribosomal protein L21 (rplU) CSB-YP421428STR
CSB-EP421428STR
CSB-BP421428STR
CSB-MP421428STR
CSB-EP421428STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 30S ribosomal protein S21 (rpsU) CSB-YP421429STR
CSB-EP421429STR
CSB-BP421429STR
CSB-MP421429STR
CSB-EP421429STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Undecaprenyl-diphosphatase (uppP), partial CSB-YP421430STR
CSB-EP421430STR
CSB-BP421430STR
CSB-MP421430STR
CSB-EP421430STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis tRNA (guanine-N (1)-)-methyltransferase CSB-YP421431STR
CSB-EP421431STR
CSB-BP421431STR
CSB-MP421431STR
CSB-EP421431STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis UPF0246 protein Ssed_1188 (Ssed_1188) CSB-YP421432STR
CSB-EP421432STR
CSB-BP421432STR
CSB-MP421432STR
CSB-EP421432STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP421433STR
CSB-EP421433STR
CSB-BP421433STR
CSB-MP421433STR
CSB-EP421433STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Enolase (eno) CSB-YP421434STR
CSB-EP421434STR
CSB-BP421434STR
CSB-MP421434STR
CSB-EP421434STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Homoserine O-succinyltransferase (metA) CSB-YP421435STR
CSB-EP421435STR
CSB-BP421435STR
CSB-MP421435STR
CSB-EP421435STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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