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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus pumilus Demethylmenaquinone methyltransferase (ubiE) CSB-YP421356BOL
CSB-EP421356BOL
CSB-BP421356BOL
CSB-MP421356BOL
CSB-EP421356BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Segregation and condensation protein A (scpA) CSB-YP421357BOL
CSB-EP421357BOL
CSB-BP421357BOL
CSB-MP421357BOL
CSB-EP421357BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP421358BOL
CSB-EP421358BOL
CSB-BP421358BOL
CSB-MP421358BOL
CSB-EP421358BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP421359BOL
CSB-EP421359BOL
CSB-BP421359BOL
CSB-MP421359BOL
CSB-EP421359BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0178 protein BPUM_2255 (BPUM_2255) CSB-YP421360BOL
CSB-EP421360BOL
CSB-BP421360BOL
CSB-MP421360BOL
CSB-EP421360BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Chaperone protein DnaK (dnaK), partial CSB-YP421361BOL
CSB-EP421361BOL
CSB-BP421361BOL
CSB-MP421361BOL
CSB-EP421361BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Uncharacterized methyltransferase BPUM_2363 (yrrT) CSB-YP421362BOL
CSB-EP421362BOL
CSB-BP421362BOL
CSB-MP421362BOL
CSB-EP421362BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP421363BOL
CSB-EP421363BOL
CSB-BP421363BOL
CSB-MP421363BOL
CSB-EP421363BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP421364BOL
CSB-EP421364BOL
CSB-BP421364BOL
CSB-MP421364BOL
CSB-EP421364BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Probable septum site-determining protein MinC (minC) CSB-YP421365BOL
CSB-EP421365BOL
CSB-BP421365BOL
CSB-MP421365BOL
CSB-EP421365BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP421366BOL
CSB-EP421366BOL
CSB-BP421366BOL
CSB-MP421366BOL
CSB-EP421366BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP421367BOL
CSB-EP421367BOL
CSB-BP421367BOL
CSB-MP421367BOL
CSB-EP421367BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Probable tRNA sulfurtransferase (thiI) CSB-YP421368BOL
CSB-EP421368BOL
CSB-BP421368BOL
CSB-MP421368BOL
CSB-EP421368BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Leucine--tRNA ligase (leuS), partial CSB-YP421369BOL
CSB-EP421369BOL
CSB-BP421369BOL
CSB-MP421369BOL
CSB-EP421369BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Biotin synthase (bioB) CSB-YP421370BOL
CSB-EP421370BOL
CSB-BP421370BOL
CSB-MP421370BOL
CSB-EP421370BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Enolase (eno) CSB-YP421371BOL
CSB-EP421371BOL
CSB-BP421371BOL
CSB-MP421371BOL
CSB-EP421371BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus ATP phosphoribosyltransferase (hisG) CSB-YP421372BOL
CSB-EP421372BOL
CSB-BP421372BOL
CSB-MP421372BOL
CSB-EP421372BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Protein translocase subunit SecA (secA), partial CSB-YP421373BOL
CSB-EP421373BOL
CSB-BP421373BOL
CSB-MP421373BOL
CSB-EP421373BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Large-conductance mechanosensitive channel (mscL), partial CSB-YP421374BOL1
CSB-EP421374BOL1
CSB-BP421374BOL1
CSB-MP421374BOL1
CSB-EP421374BOL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Uracil phosphoribosyltransferase (upp) CSB-YP421375BOL
CSB-EP421375BOL
CSB-BP421375BOL
CSB-MP421375BOL
CSB-EP421375BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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