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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Serratia proteamaculans Phosphopantetheine adenylyltransferase (coaD) CSB-YP422616STJ
CSB-EP422616STJ
CSB-BP422616STJ
CSB-MP422616STJ
CSB-EP422616STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP422617STJ
CSB-EP422617STJ
CSB-BP422617STJ
CSB-MP422617STJ
CSB-EP422617STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP422618STJ
CSB-EP422618STJ
CSB-BP422618STJ
CSB-MP422618STJ
CSB-EP422618STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari DNA replication and repair protein recF (recF) CSB-YP422619RIL
CSB-EP422619RIL
CSB-BP422619RIL
CSB-MP422619RIL
CSB-EP422619RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Deoxyguanosinetriphosphate triphosphohydrolase-like protein (A1C_00500) CSB-YP422620RIL
CSB-EP422620RIL
CSB-BP422620RIL
CSB-MP422620RIL
CSB-EP422620RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Acetate kinase (ackA) CSB-YP422621RIL
CSB-EP422621RIL
CSB-BP422621RIL
CSB-MP422621RIL
CSB-EP422621RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari NADH-quinone oxidoreductase subunit F (nuoF) CSB-YP422622RIL
CSB-EP422622RIL
CSB-BP422622RIL
CSB-MP422622RIL
CSB-EP422622RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari 30S ribosomal protein S7 (rpsG) CSB-YP422623RIL
CSB-EP422623RIL
CSB-BP422623RIL
CSB-MP422623RIL
CSB-EP422623RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari 50S ribosomal protein L7/L12 (rplL) CSB-YP422624RIL
CSB-EP422624RIL
CSB-BP422624RIL
CSB-MP422624RIL
CSB-EP422624RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP422625RIL
CSB-EP422625RIL
CSB-BP422625RIL
CSB-MP422625RIL
CSB-EP422625RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Chaperone protein DnaK (dnaK), partial CSB-YP422626RIL
CSB-EP422626RIL
CSB-BP422626RIL
CSB-MP422626RIL
CSB-EP422626RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP422627RIL
CSB-EP422627RIL
CSB-BP422627RIL
CSB-MP422627RIL
CSB-EP422627RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari RNA pyrophosphohydrolase (rppH) CSB-YP422628RIL
CSB-EP422628RIL
CSB-BP422628RIL
CSB-MP422628RIL
CSB-EP422628RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Lipid-A-disaccharide synthase (lpxB) CSB-YP422629RIL
CSB-EP422629RIL
CSB-BP422629RIL
CSB-MP422629RIL
CSB-EP422629RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Ribosome maturation factor RimM (rimM) CSB-YP422630RIL
CSB-EP422630RIL
CSB-BP422630RIL
CSB-MP422630RIL
CSB-EP422630RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP422631RIL
CSB-EP422631RIL
CSB-BP422631RIL
CSB-MP422631RIL
CSB-EP422631RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP422632RIL
CSB-EP422632RIL
CSB-BP422632RIL
CSB-MP422632RIL
CSB-EP422632RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Lipoprotein signal peptidase (lspA), partial CSB-YP422633RIL1
CSB-EP422633RIL1
CSB-BP422633RIL1
CSB-MP422633RIL1
CSB-EP422633RIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Recombination protein RecR (recR) CSB-YP422634RIL
CSB-EP422634RIL
CSB-BP422634RIL
CSB-MP422634RIL
CSB-EP422634RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Ribosome maturation factor RimP (rimP) CSB-YP422635RIL
CSB-EP422635RIL
CSB-BP422635RIL
CSB-MP422635RIL
CSB-EP422635RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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