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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O9:H4 Glucans biosynthesis protein G (mdoG) CSB-YP422036EJF
CSB-EP422036EJF
CSB-BP422036EJF
CSB-MP422036EJF
CSB-EP422036EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Flagellar P-ring protein (flgI) CSB-YP422037EJF
CSB-EP422037EJF
CSB-BP422037EJF
CSB-MP422037EJF
CSB-EP422037EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0227 protein ycfP (ycfP) CSB-YP422038EJF
CSB-EP422038EJF
CSB-BP422038EJF
CSB-MP422038EJF
CSB-EP422038EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Cell division topological specificity factor (minE) CSB-YP422039EJF
CSB-EP422039EJF
CSB-BP422039EJF
CSB-MP422039EJF
CSB-EP422039EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Cell volume regulation protein A (cvrA), partial CSB-YP422040EJF
CSB-EP422040EJF
CSB-BP422040EJF
CSB-MP422040EJF
CSB-EP422040EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0225 protein ychJ (ychJ) CSB-YP422041EJF
CSB-EP422041EJF
CSB-BP422041EJF
CSB-MP422041EJF
CSB-EP422041EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 GTP cyclohydrolase-2 (ribA) CSB-YP422042EJF
CSB-EP422042EJF
CSB-BP422042EJF
CSB-MP422042EJF
CSB-EP422042EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Autoinducer 2 kinase LsrK (lsrK) CSB-YP422043EJF
CSB-EP422043EJF
CSB-BP422043EJF
CSB-MP422043EJF
CSB-EP422043EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Trans-aconitate 2-methyltransferase (tam) CSB-YP422044EJF
CSB-EP422044EJF
CSB-BP422044EJF
CSB-MP422044EJF
CSB-EP422044EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0060 membrane protein ynfA (ynfA), partial CSB-YP422045EJF1
CSB-EP422045EJF1
CSB-BP422045EJF1
CSB-MP422045EJF1
CSB-EP422045EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 DNA replication terminus site-binding protein (tus) CSB-YP422046EJF
CSB-EP422046EJF
CSB-BP422046EJF
CSB-MP422046EJF
CSB-EP422046EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Electron transport complex protein RnfE (rnfE), partial CSB-YP422047EJF1
CSB-EP422047EJF1
CSB-BP422047EJF1
CSB-MP422047EJF1
CSB-EP422047EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Cysteine desulfuration protein sufE (sufE) CSB-YP422048EJF
CSB-EP422048EJF
CSB-BP422048EJF
CSB-MP422048EJF
CSB-EP422048EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP422049EJF
CSB-EP422049EJF
CSB-BP422049EJF
CSB-MP422049EJF
CSB-EP422049EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 N-succinylarginine dihydrolase (astB) CSB-YP422050EJF
CSB-EP422050EJF
CSB-BP422050EJF
CSB-MP422050EJF
CSB-EP422050EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP422051EJF
CSB-EP422051EJF
CSB-BP422051EJF
CSB-MP422051EJF
CSB-EP422051EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 ProP effector (proQ) CSB-YP422052EJF
CSB-EP422052EJF
CSB-BP422052EJF
CSB-MP422052EJF
CSB-EP422052EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Arginine--tRNA ligase (argS) CSB-YP422053EJF
CSB-EP422053EJF
CSB-BP422053EJF
CSB-MP422053EJF
CSB-EP422053EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP422054EJF
CSB-EP422054EJF
CSB-BP422054EJF
CSB-MP422054EJF
CSB-EP422054EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP422055EJF
CSB-EP422055EJF
CSB-BP422055EJF
CSB-MP422055EJF
CSB-EP422055EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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