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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rickettsia canadensis Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP422296RMR
CSB-EP422296RMR
CSB-BP422296RMR
CSB-MP422296RMR
CSB-EP422296RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Possible hemolysin C (tlyC) CSB-YP422297RMR
CSB-EP422297RMR
CSB-BP422297RMR
CSB-MP422297RMR
CSB-EP422297RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 50S ribosomal protein L27 (rpmA) CSB-YP422298RMR
CSB-EP422298RMR
CSB-BP422298RMR
CSB-MP422298RMR
CSB-EP422298RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP422299RMR
CSB-EP422299RMR
CSB-BP422299RMR
CSB-MP422299RMR
CSB-EP422299RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP422300RMR
CSB-EP422300RMR
CSB-BP422300RMR
CSB-MP422300RMR
CSB-EP422300RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis GTPase obg (obg) CSB-YP422301RMR
CSB-EP422301RMR
CSB-BP422301RMR
CSB-MP422301RMR
CSB-EP422301RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP422302RMR
CSB-EP422302RMR
CSB-BP422302RMR
CSB-MP422302RMR
CSB-EP422302RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Putative phosphotransferase RMA_0001 (RMA_0001) CSB-YP422303RIN
CSB-EP422303RIN
CSB-BP422303RIN
CSB-MP422303RIN
CSB-EP422303RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae 30S ribosomal protein S18 (rpsR) CSB-YP422304RIN
CSB-EP422304RIN
CSB-BP422304RIN
CSB-MP422304RIN
CSB-EP422304RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Cysteine--tRNA ligase (cysS) CSB-YP422305RIN
CSB-EP422305RIN
CSB-BP422305RIN
CSB-MP422305RIN
CSB-EP422305RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Signal peptidase I (lepB), partial CSB-YP422306RIN
CSB-EP422306RIN
CSB-BP422306RIN
CSB-MP422306RIN
CSB-EP422306RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae 50S ribosomal protein L10 (rplJ) CSB-YP422307RIN
CSB-EP422307RIN
CSB-BP422307RIN
CSB-MP422307RIN
CSB-EP422307RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Chaperone protein DnaJ (dnaJ) CSB-YP422308RIN
CSB-EP422308RIN
CSB-BP422308RIN
CSB-MP422308RIN
CSB-EP422308RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP422309RIN
CSB-EP422309RIN
CSB-BP422309RIN
CSB-MP422309RIN
CSB-EP422309RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP422310RIN
CSB-EP422310RIN
CSB-BP422310RIN
CSB-MP422310RIN
CSB-EP422310RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP422311RIN
CSB-EP422311RIN
CSB-BP422311RIN
CSB-MP422311RIN
CSB-EP422311RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Leucine--tRNA ligase (leuS), partial CSB-YP422312RIN
CSB-EP422312RIN
CSB-BP422312RIN
CSB-MP422312RIN
CSB-EP422312RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Ferredoxin--NADP reductase (RMA_0647) CSB-YP422313RIN
CSB-EP422313RIN
CSB-BP422313RIN
CSB-MP422313RIN
CSB-EP422313RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP422314RIN
CSB-EP422314RIN
CSB-BP422314RIN
CSB-MP422314RIN
CSB-EP422314RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae 50S ribosomal protein L15 (rplO) CSB-YP422315RIN
CSB-EP422315RIN
CSB-BP422315RIN
CSB-MP422315RIN
CSB-EP422315RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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