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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O9:H4 Deoxycytidine triphosphate deaminase (dcd) CSB-YP423097EJF
CSB-EP423097EJF
CSB-BP423097EJF
CSB-MP423097EJF
CSB-EP423097EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY) CSB-YP423098EJF
CSB-EP423098EJF
CSB-BP423098EJF
CSB-MP423098EJF
CSB-EP423098EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 S-formylglutathione hydrolase yeiG (yeiG) CSB-YP423099EJF
CSB-EP423099EJF
CSB-BP423099EJF
CSB-MP423099EJF
CSB-EP423099EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0352 protein yejL (yejL) CSB-YP423100EJF
CSB-EP423100EJF
CSB-BP423100EJF
CSB-MP423100EJF
CSB-EP423100EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP423101EJF
CSB-EP423101EJF
CSB-BP423101EJF
CSB-MP423101EJF
CSB-EP423101EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP423102EJF
CSB-EP423102EJF
CSB-BP423102EJF
CSB-MP423102EJF
CSB-EP423102EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP423103EJF1
CSB-EP423103EJF1
CSB-BP423103EJF1
CSB-MP423103EJF1
CSB-EP423103EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Penicillin-insensitive murein endopeptidase (mepA) CSB-YP423104EJF
CSB-EP423104EJF
CSB-BP423104EJF
CSB-MP423104EJF
CSB-EP423104EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 HTH-type transcriptional regulator murR (murR) CSB-YP423105EJF
CSB-EP423105EJF
CSB-BP423105EJF
CSB-MP423105EJF
CSB-EP423105EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP423106EJF
CSB-EP423106EJF
CSB-BP423106EJF
CSB-MP423106EJF
CSB-EP423106EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP423107EJF
CSB-EP423107EJF
CSB-BP423107EJF
CSB-MP423107EJF
CSB-EP423107EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP423108EJF
CSB-EP423108EJF
CSB-BP423108EJF
CSB-MP423108EJF
CSB-EP423108EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 3-phenylpropionate/cinnamic acid dioxygenase subunit beta (hcaF) CSB-YP423109EJF
CSB-EP423109EJF
CSB-BP423109EJF
CSB-MP423109EJF
CSB-EP423109EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 DNA repair protein recO (recO) CSB-YP423110EJF
CSB-EP423110EJF
CSB-BP423110EJF
CSB-MP423110EJF
CSB-EP423110EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 50S ribosomal protein L19 (rplS) CSB-YP423111EJF
CSB-EP423111EJF
CSB-BP423111EJF
CSB-MP423111EJF
CSB-EP423111EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protein CsiD (csiD) CSB-YP423112EJF
CSB-EP423112EJF
CSB-BP423112EJF
CSB-MP423112EJF
CSB-EP423112EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Nitric oxide reductase FlRd-NAD (+) reductase CSB-YP423113EJF
CSB-EP423113EJF
CSB-BP423113EJF
CSB-MP423113EJF
CSB-EP423113EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Adenylyl-sulfate kinase (cysC) CSB-YP423114EJF
CSB-EP423114EJF
CSB-BP423114EJF
CSB-MP423114EJF
CSB-EP423114EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Sulfite reductase [NADPH] hemoprotein beta-component (cysI) CSB-YP423115EJF
CSB-EP423115EJF
CSB-BP423115EJF
CSB-MP423115EJF
CSB-EP423115EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP423116EJF
CSB-EP423116EJF
CSB-BP423116EJF
CSB-MP423116EJF
CSB-EP423116EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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