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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus pumilus 50S ribosomal protein L30 (rpmD) CSB-YP422356BOL
CSB-EP422356BOL
CSB-BP422356BOL
CSB-MP422356BOL
CSB-EP422356BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP422357BOL
CSB-EP422357BOL
CSB-BP422357BOL
CSB-MP422357BOL
CSB-EP422357BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP422358BOL
CSB-EP422358BOL
CSB-BP422358BOL
CSB-MP422358BOL
CSB-EP422358BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP422359BOL
CSB-EP422359BOL
CSB-BP422359BOL
CSB-MP422359BOL
CSB-EP422359BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus FMN-dependent NADH-azoreductase (azoR) CSB-YP422360BOL
CSB-EP422360BOL
CSB-BP422360BOL
CSB-MP422360BOL
CSB-EP422360BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Serine--tRNA ligase (serS) CSB-YP422361BOL
CSB-EP422361BOL
CSB-BP422361BOL
CSB-MP422361BOL
CSB-EP422361BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 60 kDa chaperonin (groL), partial CSB-YP422362BOL
CSB-EP422362BOL
CSB-BP422362BOL
CSB-MP422362BOL
CSB-EP422362BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP422363BOL
CSB-EP422363BOL
CSB-BP422363BOL
CSB-MP422363BOL
CSB-EP422363BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0435 protein BPUM_0734 (BPUM_0734) CSB-YP422364BOL
CSB-EP422364BOL
CSB-BP422364BOL
CSB-MP422364BOL
CSB-EP422364BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Putative metal-dependent hydrolase BPUM_0784 (BPUM_0784) CSB-YP422365BOL
CSB-EP422365BOL
CSB-BP422365BOL
CSB-MP422365BOL
CSB-EP422365BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0295 protein BPUM_0828 (BPUM_0828), partial CSB-YP422366BOL1
CSB-EP422366BOL1
CSB-BP422366BOL1
CSB-MP422366BOL1
CSB-EP422366BOL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus HTH-type transcriptional regulator hpr (hpr) CSB-YP422367BOL
CSB-EP422367BOL
CSB-BP422367BOL
CSB-MP422367BOL
CSB-EP422367BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP422368BOL
CSB-EP422368BOL
CSB-BP422368BOL
CSB-MP422368BOL
CSB-EP422368BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0477 protein BPUM_1117 (BPUM_1117) CSB-YP422369BOL
CSB-EP422369BOL
CSB-BP422369BOL
CSB-MP422369BOL
CSB-EP422369BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 7-cyano-7-deazaguanine synthase (queC) CSB-YP422370BOL
CSB-EP422370BOL
CSB-BP422370BOL
CSB-MP422370BOL
CSB-EP422370BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Adenine deaminase (ade), partial CSB-YP422371BOL
CSB-EP422371BOL
CSB-BP422371BOL
CSB-MP422371BOL
CSB-EP422371BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP422372BOL
CSB-EP422372BOL
CSB-BP422372BOL
CSB-MP422372BOL
CSB-EP422372BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Aspartate carbamoyltransferase (pyrB) CSB-YP422373BOL
CSB-EP422373BOL
CSB-BP422373BOL
CSB-MP422373BOL
CSB-EP422373BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP422374BOL
CSB-EP422374BOL
CSB-BP422374BOL
CSB-MP422374BOL
CSB-EP422374BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP422375BOL
CSB-EP422375BOL
CSB-BP422375BOL
CSB-MP422375BOL
CSB-EP422375BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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