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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella sediminis UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP422456STR
CSB-EP422456STR
CSB-BP422456STR
CSB-MP422456STR
CSB-EP422456STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP422457STR
CSB-EP422457STR
CSB-BP422457STR
CSB-MP422457STR
CSB-EP422457STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Phosphoglycerate kinase (pgk) CSB-YP422458STR
CSB-EP422458STR
CSB-BP422458STR
CSB-MP422458STR
CSB-EP422458STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP422459STR1
CSB-EP422459STR1
CSB-BP422459STR1
CSB-MP422459STR1
CSB-EP422459STR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Multifunctional CCA protein (cca) CSB-YP422460STR
CSB-EP422460STR
CSB-BP422460STR
CSB-MP422460STR
CSB-EP422460STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Elongation factor 4 (lepA), partial CSB-YP422461STR
CSB-EP422461STR
CSB-BP422461STR
CSB-MP422461STR
CSB-EP422461STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 30S ribosomal protein S20 (rpsT) CSB-YP422462STR
CSB-EP422462STR
CSB-BP422462STR
CSB-MP422462STR
CSB-EP422462STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis tRNA (guanine-N (7)-)-methyltransferase CSB-YP422463STR
CSB-EP422463STR
CSB-BP422463STR
CSB-MP422463STR
CSB-EP422463STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP422464STR
CSB-EP422464STR
CSB-BP422464STR
CSB-MP422464STR
CSB-EP422464STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis SsrA-binding protein (smpB) CSB-YP422465STR
CSB-EP422465STR
CSB-BP422465STR
CSB-MP422465STR
CSB-EP422465STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Bifunctional protein FolD (folD) CSB-YP422466STR
CSB-EP422466STR
CSB-BP422466STR
CSB-MP422466STR
CSB-EP422466STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP422467STR
CSB-EP422467STR
CSB-BP422467STR
CSB-MP422467STR
CSB-EP422467STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Elongation factor P-like protein (Ssed_1806) CSB-YP422468STR
CSB-EP422468STR
CSB-BP422468STR
CSB-MP422468STR
CSB-EP422468STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis High frequency lysogenization protein HflD homolog (hflD) CSB-YP422469STR
CSB-EP422469STR
CSB-BP422469STR
CSB-MP422469STR
CSB-EP422469STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Thiazole synthase (thiG) CSB-YP422470STR
CSB-EP422470STR
CSB-BP422470STR
CSB-MP422470STR
CSB-EP422470STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP422471STR
CSB-EP422471STR
CSB-BP422471STR
CSB-MP422471STR
CSB-EP422471STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Outer-membrane lipoprotein carrier protein (lolA) CSB-YP422472STR
CSB-EP422472STR
CSB-BP422472STR
CSB-MP422472STR
CSB-EP422472STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Integration host factor subunit beta (ihfB) CSB-YP422473STR
CSB-EP422473STR
CSB-BP422473STR
CSB-MP422473STR
CSB-EP422473STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Deoxyguanosinetriphosphate triphosphohydrolase-like protein (Ssed_2346) CSB-YP422474STR
CSB-EP422474STR
CSB-BP422474STR
CSB-MP422474STR
CSB-EP422474STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP422475STR
CSB-EP422475STR
CSB-BP422475STR
CSB-MP422475STR
CSB-EP422475STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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