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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dinoroseobacter shibae UPF0753 protein Dshi_0997 (Dshi_0997), partial CSB-YP422857DIB
CSB-EP422857DIB
CSB-BP422857DIB
CSB-MP422857DIB
CSB-EP422857DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Integration host factor subunit beta (ihfB) CSB-YP422858DIB
CSB-EP422858DIB
CSB-BP422858DIB
CSB-MP422858DIB
CSB-EP422858DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Catalase-peroxidase (katG), partial CSB-YP422859DIB
CSB-EP422859DIB
CSB-BP422859DIB
CSB-MP422859DIB
CSB-EP422859DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Phosphoglycerate kinase (pgk) CSB-YP422860STC
CSB-EP422860STC
CSB-BP422860STC
CSB-MP422860STC
CSB-EP422860STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Tryptophan synthase alpha chain (trpA) CSB-YP422861STC
CSB-EP422861STC
CSB-BP422861STC
CSB-MP422861STC
CSB-EP422861STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP422862STC
CSB-EP422862STC
CSB-BP422862STC
CSB-MP422862STC
CSB-EP422862STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Ribonuclease P protein component (rnpA) CSB-YP422863STC
CSB-EP422863STC
CSB-BP422863STC
CSB-MP422863STC
CSB-EP422863STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola 3-dehydroquinate dehydratase (aroQ) CSB-YP422864STC
CSB-EP422864STC
CSB-BP422864STC
CSB-MP422864STC
CSB-EP422864STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP422865STC
CSB-EP422865STC
CSB-BP422865STC
CSB-MP422865STC
CSB-EP422865STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Acetylglutamate kinase (argB) CSB-YP422866STC
CSB-EP422866STC
CSB-BP422866STC
CSB-MP422866STC
CSB-EP422866STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Catalase-peroxidase (katG), partial CSB-YP422867STC
CSB-EP422867STC
CSB-BP422867STC
CSB-MP422867STC
CSB-EP422867STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Lon protease (lon), partial CSB-YP422868STC
CSB-EP422868STC
CSB-BP422868STC
CSB-MP422868STC
CSB-EP422868STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP422869STC
CSB-EP422869STC
CSB-BP422869STC
CSB-MP422869STC
CSB-EP422869STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Pup--protein ligase (pafA) CSB-YP422870STC
CSB-EP422870STC
CSB-BP422870STC
CSB-MP422870STC
CSB-EP422870STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP422871IND1
CSB-EP422871IND1
CSB-BP422871IND1
CSB-MP422871IND1
CSB-EP422871IND1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea Photosystem II D2 protein (psbD), partial CSB-YP422872IND1
CSB-EP422872IND1
CSB-BP422872IND1
CSB-MP422872IND1
CSB-EP422872IND1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea Chloroplast envelope membrane protein (cemA), partial CSB-YP422873IND1
CSB-EP422873IND1
CSB-BP422873IND1
CSB-MP422873IND1
CSB-EP422873IND1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP422874IND
CSB-EP422874IND
CSB-BP422874IND
CSB-MP422874IND
CSB-EP422874IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP422875IND
CSB-EP422875IND
CSB-BP422875IND
CSB-MP422875IND
CSB-EP422875IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea NAD (P)H-quinone oxidoreductase chain 4, chloroplastic, partial CSB-YP422876IND
CSB-EP422876IND
CSB-BP422876IND
CSB-MP422876IND
CSB-EP422876IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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