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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Citrobacter koseri 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP424257CWS
CSB-EP424257CWS
CSB-BP424257CWS
CSB-MP424257CWS
CSB-EP424257CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Probable crotonobetaine/carnitine-CoA ligase (caiC), partial CSB-YP424258CWS
CSB-EP424258CWS
CSB-BP424258CWS
CSB-MP424258CWS
CSB-EP424258CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Na (+)/H (+) antiporter nhaA, partial CSB-YP424259CWS
CSB-EP424259CWS
CSB-BP424259CWS
CSB-MP424259CWS
CSB-EP424259CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Peptide chain release factor 3 (prfC), partial CSB-YP424260CWS
CSB-EP424260CWS
CSB-BP424260CWS
CSB-MP424260CWS
CSB-EP424260CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP424261CWS
CSB-EP424261CWS
CSB-BP424261CWS
CSB-MP424261CWS
CSB-EP424261CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Elongation factor P (efp) CSB-YP424262CWS
CSB-EP424262CWS
CSB-BP424262CWS
CSB-MP424262CWS
CSB-EP424262CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Cation/acetate symporter ActP (actP), partial CSB-YP424263CWS
CSB-EP424263CWS
CSB-BP424263CWS
CSB-MP424263CWS
CSB-EP424263CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Protein RnfH (rnfH) CSB-YP424264CWS
CSB-EP424264CWS
CSB-BP424264CWS
CSB-MP424264CWS
CSB-EP424264CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri L-alanine exporter AlaE (alaE), partial CSB-YP424265CWS1
CSB-EP424265CWS1
CSB-BP424265CWS1
CSB-MP424265CWS1
CSB-EP424265CWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri DNA mismatch repair protein MutS (mutS), partial CSB-YP424266CWS
CSB-EP424266CWS
CSB-BP424266CWS
CSB-MP424266CWS
CSB-EP424266CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Cell division protein FtsB (ftsB), partial CSB-YP424267CWS
CSB-EP424267CWS
CSB-BP424267CWS
CSB-MP424267CWS
CSB-EP424267CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Nickel/cobalt efflux system rcnA (rcnA), partial CSB-YP424268CWS1
CSB-EP424268CWS1
CSB-BP424268CWS1
CSB-MP424268CWS1
CSB-EP424268CWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Cell division protein ZapA (zapA) CSB-YP424269CWS
CSB-EP424269CWS
CSB-BP424269CWS
CSB-MP424269CWS
CSB-EP424269CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri UPF0301 protein CKO_04323 (CKO_04323) CSB-YP424270CWS
CSB-EP424270CWS
CSB-BP424270CWS
CSB-MP424270CWS
CSB-EP424270CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP424271CWS
CSB-EP424271CWS
CSB-BP424271CWS
CSB-MP424271CWS
CSB-EP424271CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Urease subunit alpha (ureC), partial CSB-YP424272CWS
CSB-EP424272CWS
CSB-BP424272CWS
CSB-MP424272CWS
CSB-EP424272CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP424273CWS
CSB-EP424273CWS
CSB-BP424273CWS
CSB-MP424273CWS
CSB-EP424273CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri D-tagatose-1,6-bisphosphate aldolase subunit kbaZ (kbaZ) CSB-YP424274CWS
CSB-EP424274CWS
CSB-BP424274CWS
CSB-MP424274CWS
CSB-EP424274CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Ribosome-binding factor A (rbfA) CSB-YP424275CWS
CSB-EP424275CWS
CSB-BP424275CWS
CSB-MP424275CWS
CSB-EP424275CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Phosphoglucosamine mutase (glmM) CSB-YP424276CWS
CSB-EP424276CWS
CSB-BP424276CWS
CSB-MP424276CWS
CSB-EP424276CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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