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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Serratia proteamaculans Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP423617STJ
CSB-EP423617STJ
CSB-BP423617STJ
CSB-MP423617STJ
CSB-EP423617STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Integration host factor subunit beta (ihfB) CSB-YP423618STJ
CSB-EP423618STJ
CSB-BP423618STJ
CSB-MP423618STJ
CSB-EP423618STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Asparagine--tRNA ligase (asnS) CSB-YP423619STJ
CSB-EP423619STJ
CSB-BP423619STJ
CSB-MP423619STJ
CSB-EP423619STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Dihydroorotate dehydrogenase (quinone) CSB-YP423620STJ
CSB-EP423620STJ
CSB-BP423620STJ
CSB-MP423620STJ
CSB-EP423620STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP423621STJ
CSB-EP423621STJ
CSB-BP423621STJ
CSB-MP423621STJ
CSB-EP423621STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB (rutB) CSB-YP423622STJ
CSB-EP423622STJ
CSB-BP423622STJ
CSB-MP423622STJ
CSB-EP423622STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Penicillin-binding protein activator LpoB (lpoB) CSB-YP423623STJ
CSB-EP423623STJ
CSB-BP423623STJ
CSB-MP423623STJ
CSB-EP423623STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Glutamyl-tRNA reductase (hemA) CSB-YP423624STJ
CSB-EP423624STJ
CSB-BP423624STJ
CSB-MP423624STJ
CSB-EP423624STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans High frequency lysogenization protein HflD homolog (hflD) CSB-YP423625STJ
CSB-EP423625STJ
CSB-BP423625STJ
CSB-MP423625STJ
CSB-EP423625STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L35 (rpmI) CSB-YP423626STJ
CSB-EP423626STJ
CSB-BP423626STJ
CSB-MP423626STJ
CSB-EP423626STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP423627STJ
CSB-EP423627STJ
CSB-BP423627STJ
CSB-MP423627STJ
CSB-EP423627STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP423628STJ1
CSB-EP423628STJ1
CSB-BP423628STJ1
CSB-MP423628STJ1
CSB-EP423628STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Dipeptide and tripeptide permease A (dtpA), partial CSB-YP423629STJ
CSB-EP423629STJ
CSB-BP423629STJ
CSB-MP423629STJ
CSB-EP423629STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0114 protein Spro_2386 (Spro_2386), partial CSB-YP423630STJ1
CSB-EP423630STJ1
CSB-BP423630STJ1
CSB-MP423630STJ1
CSB-EP423630STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans GTP cyclohydrolase folE2 (folE2) CSB-YP423631STJ
CSB-EP423631STJ
CSB-BP423631STJ
CSB-MP423631STJ
CSB-EP423631STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP423632STJ
CSB-EP423632STJ
CSB-BP423632STJ
CSB-MP423632STJ
CSB-EP423632STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Ribonuclease D (rnd) CSB-YP423633STJ
CSB-EP423633STJ
CSB-BP423633STJ
CSB-MP423633STJ
CSB-EP423633STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP423634STJ
CSB-EP423634STJ
CSB-BP423634STJ
CSB-MP423634STJ
CSB-EP423634STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0502 protein Spro_2794 (Spro_2794) CSB-YP423635STJ
CSB-EP423635STJ
CSB-BP423635STJ
CSB-MP423635STJ
CSB-EP423635STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Flagellar protein FliT (fliT) CSB-YP423636STJ
CSB-EP423636STJ
CSB-BP423636STJ
CSB-MP423636STJ
CSB-EP423636STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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