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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rickettsia rickettsii Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP422676RNC
CSB-EP422676RNC
CSB-BP422676RNC
CSB-MP422676RNC
CSB-EP422676RNC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia rickettsii Octanoyltransferase (lipB) CSB-YP422677RNC
CSB-EP422677RNC
CSB-BP422677RNC
CSB-MP422677RNC
CSB-EP422677RNC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Putative Holliday junction resolvase (A1I_05165) CSB-YP422678RIM
CSB-EP422678RIM
CSB-BP422678RIM
CSB-MP422678RIM
CSB-EP422678RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP422679RIM1
CSB-EP422679RIM1
CSB-BP422679RIM1
CSB-MP422679RIM1
CSB-EP422679RIM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Probable rRNA maturation factor (A1I_00310) CSB-YP422680RIM
CSB-EP422680RIM
CSB-BP422680RIM
CSB-MP422680RIM
CSB-EP422680RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Protein-export protein secB (secB) CSB-YP422681RIM
CSB-EP422681RIM
CSB-BP422681RIM
CSB-MP422681RIM
CSB-EP422681RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Cysteine--tRNA ligase (cysS) CSB-YP422682RIM
CSB-EP422682RIM
CSB-BP422682RIM
CSB-MP422682RIM
CSB-EP422682RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP422683RIM
CSB-EP422683RIM
CSB-BP422683RIM
CSB-MP422683RIM
CSB-EP422683RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Recombination protein RecR (recR) CSB-YP422684RIM
CSB-EP422684RIM
CSB-BP422684RIM
CSB-MP422684RIM
CSB-EP422684RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP422685RIM
CSB-EP422685RIM
CSB-BP422685RIM
CSB-MP422685RIM
CSB-EP422685RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii 50S ribosomal protein L1 (rplA) CSB-YP422686RIM
CSB-EP422686RIM
CSB-BP422686RIM
CSB-MP422686RIM
CSB-EP422686RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP422687RIM
CSB-EP422687RIM
CSB-BP422687RIM
CSB-MP422687RIM
CSB-EP422687RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii UvrABC system protein B (uvrB), partial CSB-YP422688RIM
CSB-EP422688RIM
CSB-BP422688RIM
CSB-MP422688RIM
CSB-EP422688RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii 30S ribosomal protein S3 (rpsC) CSB-YP422689RIM
CSB-EP422689RIM
CSB-BP422689RIM
CSB-MP422689RIM
CSB-EP422689RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii 30S ribosomal protein S11 (rpsK) CSB-YP422690RIM
CSB-EP422690RIM
CSB-BP422690RIM
CSB-MP422690RIM
CSB-EP422690RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Protein translocase subunit SecA (secA), partial CSB-YP422691RIM
CSB-EP422691RIM
CSB-BP422691RIM
CSB-MP422691RIM
CSB-EP422691RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii 50S ribosomal protein L20 (rplT) CSB-YP422692RIM
CSB-EP422692RIM
CSB-BP422692RIM
CSB-MP422692RIM
CSB-EP422692RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii 30S ribosomal protein S4 (rpsD) CSB-YP422693RIM
CSB-EP422693RIM
CSB-BP422693RIM
CSB-MP422693RIM
CSB-EP422693RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Co-chaperone protein hscB homolog (hscB) CSB-YP422694RIM
CSB-EP422694RIM
CSB-BP422694RIM
CSB-MP422694RIM
CSB-EP422694RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Probable transcriptional regulatory protein A1I_03240 (A1I_03240) CSB-YP422695RIM
CSB-EP422695RIM
CSB-BP422695RIM
CSB-MP422695RIM
CSB-EP422695RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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