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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O9:H4 Dihydroorotate dehydrogenase (quinone) CSB-YP423077EJF
CSB-EP423077EJF
CSB-BP423077EJF
CSB-MP423077EJF
CSB-EP423077EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Chaperone protein TorD (torD) CSB-YP423078EJF
CSB-EP423078EJF
CSB-BP423078EJF
CSB-MP423078EJF
CSB-EP423078EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB (rutB) CSB-YP423079EJF
CSB-EP423079EJF
CSB-BP423079EJF
CSB-MP423079EJF
CSB-EP423079EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 N-methyl-L-tryptophan oxidase (solA) CSB-YP423080EJF
CSB-EP423080EJF
CSB-BP423080EJF
CSB-MP423080EJF
CSB-EP423080EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Phosphate acyltransferase (plsX) CSB-YP423081EJF
CSB-EP423081EJF
CSB-BP423081EJF
CSB-MP423081EJF
CSB-EP423081EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Peptidase T (pepT) CSB-YP423082EJF
CSB-EP423082EJF
CSB-BP423082EJF
CSB-MP423082EJF
CSB-EP423082EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Na (+)/H (+) antiporter nhaB, partial CSB-YP423083EJF
CSB-EP423083EJF
CSB-BP423083EJF
CSB-MP423083EJF
CSB-EP423083EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Outer-membrane lipoprotein lolB (lolB) CSB-YP423084EJF
CSB-EP423084EJF
CSB-BP423084EJF
CSB-MP423084EJF
CSB-EP423084EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0259 membrane protein yciC (yciC), partial CSB-YP423085EJF1
CSB-EP423085EJF1
CSB-BP423085EJF1
CSB-MP423085EJF1
CSB-EP423085EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP423086EJF
CSB-EP423086EJF
CSB-BP423086EJF
CSB-MP423086EJF
CSB-EP423086EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Transcriptional regulator lsrR (lsrR) CSB-YP423087EJF
CSB-EP423087EJF
CSB-BP423087EJF
CSB-MP423087EJF
CSB-EP423087EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Probable sugar efflux transporter (sotB), partial CSB-YP423088EJF
CSB-EP423088EJF
CSB-BP423088EJF
CSB-MP423088EJF
CSB-EP423088EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Dihydrofolate reductase folM (folM) CSB-YP423089EJF
CSB-EP423089EJF
CSB-BP423089EJF
CSB-MP423089EJF
CSB-EP423089EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP423090EJF
CSB-EP423090EJF
CSB-BP423090EJF
CSB-MP423090EJF
CSB-EP423090EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 3-dehydroquinate dehydratase (aroD) CSB-YP423091EJF
CSB-EP423091EJF
CSB-BP423091EJF
CSB-MP423091EJF
CSB-EP423091EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Integration host factor subunit alpha (ihfA) CSB-YP423092EJF
CSB-EP423092EJF
CSB-BP423092EJF
CSB-MP423092EJF
CSB-EP423092EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protein ves (ves) CSB-YP423093EJF
CSB-EP423093EJF
CSB-BP423093EJF
CSB-MP423093EJF
CSB-EP423093EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP423094EJF
CSB-EP423094EJF
CSB-BP423094EJF
CSB-MP423094EJF
CSB-EP423094EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UvrABC system protein C (uvrC) CSB-YP423095EJF
CSB-EP423095EJF
CSB-BP423095EJF
CSB-MP423095EJF
CSB-EP423095EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP423096EJF1
CSB-EP423096EJF1
CSB-BP423096EJF1
CSB-MP423096EJF1
CSB-EP423096EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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