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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dinoroseobacter shibae DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP422837DIB
CSB-EP422837DIB
CSB-BP422837DIB
CSB-MP422837DIB
CSB-EP422837DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae UPF0042 nucleotide-binding protein Dshi_0209 (Dshi_0209) CSB-YP422838DIB
CSB-EP422838DIB
CSB-BP422838DIB
CSB-MP422838DIB
CSB-EP422838DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Large-conductance mechanosensitive channel (mscL), partial CSB-YP422839DIB1
CSB-EP422839DIB1
CSB-BP422839DIB1
CSB-MP422839DIB1
CSB-EP422839DIB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Periplasmic nitrate reductase (napA), partial CSB-YP422840DIB
CSB-EP422840DIB
CSB-BP422840DIB
CSB-MP422840DIB
CSB-EP422840DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae 50S ribosomal protein L16 (rplP) CSB-YP422841DIB
CSB-EP422841DIB
CSB-BP422841DIB
CSB-MP422841DIB
CSB-EP422841DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae 50S ribosomal protein L24 (rplX) CSB-YP422842DIB
CSB-EP422842DIB
CSB-BP422842DIB
CSB-MP422842DIB
CSB-EP422842DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae 50S ribosomal protein L15 (rplO) CSB-YP422843DIB
CSB-EP422843DIB
CSB-BP422843DIB
CSB-MP422843DIB
CSB-EP422843DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae 50S ribosomal protein L34 (rpmH) CSB-YP422844DIB
CSB-EP422844DIB
CSB-BP422844DIB
CSB-MP422844DIB
CSB-EP422844DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP422845DIB
CSB-EP422845DIB
CSB-BP422845DIB
CSB-MP422845DIB
CSB-EP422845DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP422846DIB
CSB-EP422846DIB
CSB-BP422846DIB
CSB-MP422846DIB
CSB-EP422846DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae ATP synthase subunit alpha 1 (atpA1), partial CSB-YP422847DIB
CSB-EP422847DIB
CSB-BP422847DIB
CSB-MP422847DIB
CSB-EP422847DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Probable transaldolase (tal) CSB-YP422848DIB
CSB-EP422848DIB
CSB-BP422848DIB
CSB-MP422848DIB
CSB-EP422848DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Shikimate dehydrogenase (aroE) CSB-YP422849DIB
CSB-EP422849DIB
CSB-BP422849DIB
CSB-MP422849DIB
CSB-EP422849DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Light-independent protochlorophyllide reductase subunit B (bchB), partial CSB-YP422850DIB
CSB-EP422850DIB
CSB-BP422850DIB
CSB-MP422850DIB
CSB-EP422850DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Enolase (eno) CSB-YP422851DIB
CSB-EP422851DIB
CSB-BP422851DIB
CSB-MP422851DIB
CSB-EP422851DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Elongation factor 4 (lepA), partial CSB-YP422852DIB
CSB-EP422852DIB
CSB-BP422852DIB
CSB-MP422852DIB
CSB-EP422852DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Urease subunit beta (ureB) CSB-YP422853DIB
CSB-EP422853DIB
CSB-BP422853DIB
CSB-MP422853DIB
CSB-EP422853DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Octanoyltransferase (lipB) CSB-YP422854DIB
CSB-EP422854DIB
CSB-BP422854DIB
CSB-MP422854DIB
CSB-EP422854DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Translation initiation factor IF-1 (infA) CSB-YP422855DIB
CSB-EP422855DIB
CSB-BP422855DIB
CSB-MP422855DIB
CSB-EP422855DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae D-alanine--D-alanine ligase (ddl) CSB-YP422856DIB
CSB-EP422856DIB
CSB-BP422856DIB
CSB-MP422856DIB
CSB-EP422856DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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