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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O9:H4 DNA replication and repair protein recF (recF) CSB-YP423137EJF
CSB-EP423137EJF
CSB-BP423137EJF
CSB-MP423137EJF
CSB-EP423137EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 tRNA modification GTPase MnmE (mnmE) CSB-YP423138EJF
CSB-EP423138EJF
CSB-BP423138EJF
CSB-MP423138EJF
CSB-EP423138EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Bifunctional protein GlmU (glmU) CSB-YP423139EJF
CSB-EP423139EJF
CSB-BP423139EJF
CSB-MP423139EJF
CSB-EP423139EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 D-ribose pyranase (rbsD) CSB-YP423140EJF
CSB-EP423140EJF
CSB-BP423140EJF
CSB-MP423140EJF
CSB-EP423140EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Tyrosine recombinase XerC (xerC) CSB-YP423141EJF
CSB-EP423141EJF
CSB-BP423141EJF
CSB-MP423141EJF
CSB-EP423141EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Probable GTP-binding protein EngB (engB) CSB-YP423142EJF
CSB-EP423142EJF
CSB-BP423142EJF
CSB-MP423142EJF
CSB-EP423142EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Rhamnulokinase (rhaB) CSB-YP423143EJF
CSB-EP423143EJF
CSB-BP423143EJF
CSB-MP423143EJF
CSB-EP423143EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 50S ribosomal protein L31 (rpmE) CSB-YP423144EJF
CSB-EP423144EJF
CSB-BP423144EJF
CSB-MP423144EJF
CSB-EP423144EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Elongation factor Tu 2 (tuf2) CSB-YP423145EJF
CSB-EP423145EJF
CSB-BP423145EJF
CSB-MP423145EJF
CSB-EP423145EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Phosphomethylpyrimidine synthase (thiC) CSB-YP423146EJF
CSB-EP423146EJF
CSB-BP423146EJF
CSB-MP423146EJF
CSB-EP423146EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Isocitrate dehydrogenase kinase/phosphatase (aceK) CSB-YP423147EJF
CSB-EP423147EJF
CSB-BP423147EJF
CSB-MP423147EJF
CSB-EP423147EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Cytochrome c-552 (nrfA) CSB-YP423148EJF
CSB-EP423148EJF
CSB-BP423148EJF
CSB-MP423148EJF
CSB-EP423148EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Uncharacterized protein YjeA (yjeA) CSB-YP423149EJF
CSB-EP423149EJF
CSB-BP423149EJF
CSB-MP423149EJF
CSB-EP423149EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protein hfq (hfq) CSB-YP423150EJF
CSB-EP423150EJF
CSB-BP423150EJF
CSB-MP423150EJF
CSB-EP423150EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 30S ribosomal protein S6 (rpsF) CSB-YP423151EJF
CSB-EP423151EJF
CSB-BP423151EJF
CSB-MP423151EJF
CSB-EP423151EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP423152EJF
CSB-EP423152EJF
CSB-BP423152EJF
CSB-MP423152EJF
CSB-EP423152EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Anti-adapter protein iraD (iraD) CSB-YP423153EJF
CSB-EP423153EJF
CSB-BP423153EJF
CSB-MP423153EJF
CSB-EP423153EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Phosphopentomutase (deoB) CSB-YP423154EJF
CSB-EP423154EJF
CSB-BP423154EJF
CSB-MP423154EJF
CSB-EP423154EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP423155EJF
CSB-EP423155EJF
CSB-BP423155EJF
CSB-MP423155EJF
CSB-EP423155EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis UPF0278 protein Igni_0092 (Igni_0092) CSB-YP423156ILH
CSB-EP423156ILH
CSB-BP423156ILH
CSB-MP423156ILH
CSB-EP423156ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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