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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O9:H4 L-rhamnose mutarotase (rhaM) CSB-YP425219EJF
CSB-EP425219EJF
CSB-BP425219EJF
CSB-MP425219EJF
CSB-EP425219EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glycerol kinase (glpK) CSB-YP425220EJF
CSB-EP425220EJF
CSB-BP425220EJF
CSB-MP425220EJF
CSB-EP425220EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Acetylglutamate kinase (argB) CSB-YP425221EJF
CSB-EP425221EJF
CSB-BP425221EJF
CSB-MP425221EJF
CSB-EP425221EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Pantothenate kinase (coaA) CSB-YP425222EJF
CSB-EP425222EJF
CSB-BP425222EJF
CSB-MP425222EJF
CSB-EP425222EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP425223EJF
CSB-EP425223EJF
CSB-BP425223EJF
CSB-MP425223EJF
CSB-EP425223EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP425224EJF
CSB-EP425224EJF
CSB-BP425224EJF
CSB-MP425224EJF
CSB-EP425224EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 10 kDa chaperonin (groS) CSB-YP425225EJF
CSB-EP425225EJF
CSB-BP425225EJF
CSB-MP425225EJF
CSB-EP425225EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (ulaD) CSB-YP425226EJF
CSB-EP425226EJF
CSB-BP425226EJF
CSB-MP425226EJF
CSB-EP425226EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Probable cytosol aminopeptidase (pepA) CSB-YP425227EJF
CSB-EP425227EJF
CSB-BP425227EJF
CSB-MP425227EJF
CSB-EP425227EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP425228EJF
CSB-EP425228EJF
CSB-BP425228EJF
CSB-MP425228EJF
CSB-EP425228EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Trp operon repressor (trpR) CSB-YP425229EJF
CSB-EP425229EJF
CSB-BP425229EJF
CSB-MP425229EJF
CSB-EP425229EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis UPF0095 protein Igni_0061 (Igni_0061) CSB-YP425230ILH
CSB-EP425230ILH
CSB-BP425230ILH
CSB-MP425230ILH
CSB-EP425230ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Non-canonical purine NTP pyrophosphatase (Igni_0179) CSB-YP425231ILH
CSB-EP425231ILH
CSB-BP425231ILH
CSB-MP425231ILH
CSB-EP425231ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis 30S ribosomal protein S12P (rps12p) CSB-YP425232ILH
CSB-EP425232ILH
CSB-BP425232ILH
CSB-MP425232ILH
CSB-EP425232ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis tRNA (Ile2) 2-agmatinylcytidine synthetase TiaS CSB-YP425233ILH
CSB-EP425233ILH
CSB-BP425233ILH
CSB-MP425233ILH
CSB-EP425233ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Probable peroxiredoxin (Igni_0459) CSB-YP425234ILH
CSB-EP425234ILH
CSB-BP425234ILH
CSB-MP425234ILH
CSB-EP425234ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis 50S ribosomal protein L22P (rpl22p) CSB-YP425235ILH
CSB-EP425235ILH
CSB-BP425235ILH
CSB-MP425235ILH
CSB-EP425235ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis V-type ATP synthase beta chain (atpB) CSB-YP425236ILH
CSB-EP425236ILH
CSB-BP425236ILH
CSB-MP425236ILH
CSB-EP425236ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis ATP phosphoribosyltransferase (hisG) CSB-YP425237ILH
CSB-EP425237ILH
CSB-BP425237ILH
CSB-MP425237ILH
CSB-EP425237ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP425238ILH
CSB-EP425238ILH
CSB-BP425238ILH
CSB-MP425238ILH
CSB-EP425238ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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