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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Serratia proteamaculans Phosphoglucosamine mutase (glmM) CSB-YP424618STJ
CSB-EP424618STJ
CSB-BP424618STJ
CSB-MP424618STJ
CSB-EP424618STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0306 protein Spro_0510 (Spro_0510) CSB-YP424619STJ
CSB-EP424619STJ
CSB-BP424619STJ
CSB-MP424619STJ
CSB-EP424619STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Peptide chain release factor 3 (prfC), partial CSB-YP424620STJ
CSB-EP424620STJ
CSB-BP424620STJ
CSB-MP424620STJ
CSB-EP424620STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP424621STJ
CSB-EP424621STJ
CSB-BP424621STJ
CSB-MP424621STJ
CSB-EP424621STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Na (+)/H (+) antiporter nhaA, partial CSB-YP424622STJ
CSB-EP424622STJ
CSB-BP424622STJ
CSB-MP424622STJ
CSB-EP424622STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP424623STJ
CSB-EP424623STJ
CSB-BP424623STJ
CSB-MP424623STJ
CSB-EP424623STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 2-isopropylmalate synthase (leuA), partial CSB-YP424624STJ
CSB-EP424624STJ
CSB-BP424624STJ
CSB-MP424624STJ
CSB-EP424624STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0289 protein Spro_0773 (Spro_0773) CSB-YP424625STJ
CSB-EP424625STJ
CSB-BP424625STJ
CSB-MP424625STJ
CSB-EP424625STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP424626STJ
CSB-EP424626STJ
CSB-BP424626STJ
CSB-MP424626STJ
CSB-EP424626STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 5'/3'-nucleotidase SurE (surE) CSB-YP424627STJ
CSB-EP424627STJ
CSB-BP424627STJ
CSB-MP424627STJ
CSB-EP424627STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 30S ribosomal protein S16 (rpsP) CSB-YP424628STJ
CSB-EP424628STJ
CSB-BP424628STJ
CSB-MP424628STJ
CSB-EP424628STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Methylthioribose kinase (mtnK) CSB-YP424629STJ
CSB-EP424629STJ
CSB-BP424629STJ
CSB-MP424629STJ
CSB-EP424629STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Glutamate 5-kinase (proB) CSB-YP424630STJ
CSB-EP424630STJ
CSB-BP424630STJ
CSB-MP424630STJ
CSB-EP424630STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans tRNA sulfurtransferase (thiI) CSB-YP424631STJ
CSB-EP424631STJ
CSB-BP424631STJ
CSB-MP424631STJ
CSB-EP424631STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans HMP-PP phosphatase (cof) CSB-YP424632STJ
CSB-EP424632STJ
CSB-BP424632STJ
CSB-MP424632STJ
CSB-EP424632STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L31 type B (rpmE2) CSB-YP424633STJ
CSB-EP424633STJ
CSB-BP424633STJ
CSB-MP424633STJ
CSB-EP424633STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Chaperone protein htpG (htpG), partial CSB-YP424634STJ
CSB-EP424634STJ
CSB-BP424634STJ
CSB-MP424634STJ
CSB-EP424634STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Leucine--tRNA ligase (leuS) CSB-YP424635STJ
CSB-EP424635STJ
CSB-BP424635STJ
CSB-MP424635STJ
CSB-EP424635STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0271 protein Spro_1257 (Spro_1257) CSB-YP424636STJ
CSB-EP424636STJ
CSB-BP424636STJ
CSB-MP424636STJ
CSB-EP424636STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP424637STJ
CSB-EP424637STJ
CSB-BP424637STJ
CSB-MP424637STJ
CSB-EP424637STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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