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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus Probable rRNA maturation factor (P9215_02001) CSB-YP425599PZD
CSB-EP425599PZD
CSB-BP425599PZD
CSB-MP425599PZD
CSB-EP425599PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L1 (rplA) CSB-YP425600PZD
CSB-EP425600PZD
CSB-BP425600PZD
CSB-MP425600PZD
CSB-EP425600PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP425601PZD
CSB-EP425601PZD
CSB-BP425601PZD
CSB-MP425601PZD
CSB-EP425601PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein H (psbH), partial CSB-YP425602PZD1
CSB-EP425602PZD1
CSB-BP425602PZD1
CSB-MP425602PZD1
CSB-EP425602PZD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP425603PZD
CSB-EP425603PZD
CSB-BP425603PZD
CSB-MP425603PZD
CSB-EP425603PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit 3, partial CSB-YP425604PZD1
CSB-EP425604PZD1
CSB-BP425604PZD1
CSB-MP425604PZD1
CSB-EP425604PZD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein T (psbT), partial CSB-YP425605PZD
CSB-EP425605PZD
CSB-BP425605PZD
CSB-MP425605PZD
CSB-EP425605PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP425606PZD
CSB-EP425606PZD
CSB-BP425606PZD
CSB-MP425606PZD
CSB-EP425606PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP425607PZD
CSB-EP425607PZD
CSB-BP425607PZD
CSB-MP425607PZD
CSB-EP425607PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP425608PZD
CSB-EP425608PZD
CSB-BP425608PZD
CSB-MP425608PZD
CSB-EP425608PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP425609PZD
CSB-EP425609PZD
CSB-BP425609PZD
CSB-MP425609PZD
CSB-EP425609PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Uridylate kinase (pyrH) CSB-YP425610PZD
CSB-EP425610PZD
CSB-BP425610PZD
CSB-MP425610PZD
CSB-EP425610PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP phosphoribosyltransferase (hisG) CSB-YP425611PZD
CSB-EP425611PZD
CSB-BP425611PZD
CSB-MP425611PZD
CSB-EP425611PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glycogen synthase (glgA) CSB-YP425612PZD
CSB-EP425612PZD
CSB-BP425612PZD
CSB-MP425612PZD
CSB-EP425612PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Putative Holliday junction resolvase (P9215_08861) CSB-YP425613PZD
CSB-EP425613PZD
CSB-BP425613PZD
CSB-MP425613PZD
CSB-EP425613PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Urease subunit alpha (ureC), partial CSB-YP425614PZD
CSB-EP425614PZD
CSB-BP425614PZD
CSB-MP425614PZD
CSB-EP425614PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP425615PZD
CSB-EP425615PZD
CSB-BP425615PZD
CSB-MP425615PZD
CSB-EP425615PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Leucine--tRNA ligase (leuS), partial CSB-YP425616PZD
CSB-EP425616PZD
CSB-BP425616PZD
CSB-MP425616PZD
CSB-EP425616PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Adaptive-response sensory-kinase sasA (sasA) CSB-YP425617PZD
CSB-EP425617PZD
CSB-BP425617PZD
CSB-MP425617PZD
CSB-EP425617PZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem Q (B) protein, partial CSB-YP425618PZD1
CSB-EP425618PZD1
CSB-BP425618PZD1
CSB-MP425618PZD1
CSB-EP425618PZD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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