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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Serratia proteamaculans Diaminopimelate epimerase (dapF) CSB-YP425639STJ
CSB-EP425639STJ
CSB-BP425639STJ
CSB-MP425639STJ
CSB-EP425639STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L10 (rplJ) CSB-YP425640STJ
CSB-EP425640STJ
CSB-BP425640STJ
CSB-MP425640STJ
CSB-EP425640STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Uncharacterized protein YjeA (yjeA) CSB-YP425641STJ
CSB-EP425641STJ
CSB-BP425641STJ
CSB-MP425641STJ
CSB-EP425641STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans HTH-type transcriptional repressor NsrR (nsrR) CSB-YP425642STJ
CSB-EP425642STJ
CSB-BP425642STJ
CSB-MP425642STJ
CSB-EP425642STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP425643STJ
CSB-EP425643STJ
CSB-BP425643STJ
CSB-MP425643STJ
CSB-EP425643STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP425644STJ
CSB-EP425644STJ
CSB-BP425644STJ
CSB-MP425644STJ
CSB-EP425644STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Deoxyribose-phosphate aldolase (deoC) CSB-YP425645STJ
CSB-EP425645STJ
CSB-BP425645STJ
CSB-MP425645STJ
CSB-EP425645STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Homoserine kinase (thrB) CSB-YP425646STJ
CSB-EP425646STJ
CSB-BP425646STJ
CSB-MP425646STJ
CSB-EP425646STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Lipoprotein signal peptidase (lspA), partial CSB-YP425647STJ1
CSB-EP425647STJ1
CSB-BP425647STJ1
CSB-MP425647STJ1
CSB-EP425647STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP425648STJ
CSB-EP425648STJ
CSB-BP425648STJ
CSB-MP425648STJ
CSB-EP425648STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein translocase subunit SecA (secA), partial CSB-YP425649STJ
CSB-EP425649STJ
CSB-BP425649STJ
CSB-MP425649STJ
CSB-EP425649STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP425650STJ
CSB-EP425650STJ
CSB-BP425650STJ
CSB-MP425650STJ
CSB-EP425650STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein RecA (recA) CSB-YP425651STJ
CSB-EP425651STJ
CSB-BP425651STJ
CSB-MP425651STJ
CSB-EP425651STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Na (+)-translocating NADH-quinone reductase subunit A CSB-YP425652STJ
CSB-EP425652STJ
CSB-BP425652STJ
CSB-MP425652STJ
CSB-EP425652STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0255 protein Spro_0965 (Spro_0965) CSB-YP425653STJ
CSB-EP425653STJ
CSB-BP425653STJ
CSB-MP425653STJ
CSB-EP425653STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP425654STJ
CSB-EP425654STJ
CSB-BP425654STJ
CSB-MP425654STJ
CSB-EP425654STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP425655STJ
CSB-EP425655STJ
CSB-BP425655STJ
CSB-MP425655STJ
CSB-EP425655STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0234 protein Spro_1084 (Spro_1084) CSB-YP425656STJ
CSB-EP425656STJ
CSB-BP425656STJ
CSB-MP425656STJ
CSB-EP425656STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Adenine deaminase (ade), partial CSB-YP425657STJ
CSB-EP425657STJ
CSB-BP425657STJ
CSB-MP425657STJ
CSB-EP425657STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP425658STJ
CSB-EP425658STJ
CSB-BP425658STJ
CSB-MP425658STJ
CSB-EP425658STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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