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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rickettsia bellii NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP425799RIM
CSB-EP425799RIM
CSB-BP425799RIM
CSB-MP425799RIM
CSB-EP425799RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Heme A synthase (ctaA), partial CSB-YP425800RIM
CSB-EP425800RIM
CSB-BP425800RIM
CSB-MP425800RIM
CSB-EP425800RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP425801RIM
CSB-EP425801RIM
CSB-BP425801RIM
CSB-MP425801RIM
CSB-EP425801RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP425802RIM
CSB-EP425802RIM
CSB-BP425802RIM
CSB-MP425802RIM
CSB-EP425802RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii 30S ribosomal protein S6 (rpsF) CSB-YP425803RIM
CSB-EP425803RIM
CSB-BP425803RIM
CSB-MP425803RIM
CSB-EP425803RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP425804RIM
CSB-EP425804RIM
CSB-BP425804RIM
CSB-MP425804RIM
CSB-EP425804RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia bellii 50S ribosomal protein L21 (rplU) CSB-YP425805RIM
CSB-EP425805RIM
CSB-BP425805RIM
CSB-MP425805RIM
CSB-EP425805RIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Sulfurtransferase TusA homolog (tusA) CSB-YP425806STP
CSB-EP425806STP
CSB-BP425806STP
CSB-MP425806STP
CSB-EP425806STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP425807STP
CSB-EP425807STP
CSB-BP425807STP
CSB-MP425807STP
CSB-EP425807STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP425808STP
CSB-EP425808STP
CSB-BP425808STP
CSB-MP425808STP
CSB-EP425808STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP425809STP
CSB-EP425809STP
CSB-BP425809STP
CSB-MP425809STP
CSB-EP425809STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 50S ribosomal protein L4 (rplD) CSB-YP425810STP
CSB-EP425810STP
CSB-BP425810STP
CSB-MP425810STP
CSB-EP425810STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 30S ribosomal protein S19 (rpsS) CSB-YP425811STP
CSB-EP425811STP
CSB-BP425811STP
CSB-MP425811STP
CSB-EP425811STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 50S ribosomal protein L24 (rplX) CSB-YP425812STP
CSB-EP425812STP
CSB-BP425812STP
CSB-MP425812STP
CSB-EP425812STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 50S ribosomal protein L18 (rplR) CSB-YP425813STP
CSB-EP425813STP
CSB-BP425813STP
CSB-MP425813STP
CSB-EP425813STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana 30S ribosomal protein S11 (rpsK) CSB-YP425814STP
CSB-EP425814STP
CSB-BP425814STP
CSB-MP425814STP
CSB-EP425814STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP425815STP
CSB-EP425815STP
CSB-BP425815STP
CSB-MP425815STP
CSB-EP425815STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP425816STP
CSB-EP425816STP
CSB-BP425816STP
CSB-MP425816STP
CSB-EP425816STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana Lysine--tRNA ligase (lysS) CSB-YP425817STP
CSB-EP425817STP
CSB-BP425817STP
CSB-MP425817STP
CSB-EP425817STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella pealeana tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP425818STP
CSB-EP425818STP
CSB-BP425818STP
CSB-MP425818STP
CSB-EP425818STP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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