Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Azorhizobium caulinodans Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP425859DPR
CSB-EP425859DPR
CSB-BP425859DPR
CSB-MP425859DPR
CSB-EP425859DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP425860DPR
CSB-EP425860DPR
CSB-BP425860DPR
CSB-MP425860DPR
CSB-EP425860DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans UPF0391 membrane protein AZC_4184 (AZC_4184), partial CSB-YP425861DPR
CSB-EP425861DPR
CSB-BP425861DPR
CSB-MP425861DPR
CSB-EP425861DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phycocyanobilin lyase subunit CpcS (cpcS) CSB-YP425862FQA
CSB-EP425862FQA
CSB-BP425862FQA
CSB-MP425862FQA
CSB-EP425862FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans 50S ribosomal protein L10 (rplJ) CSB-YP425863DPR
CSB-EP425863DPR
CSB-BP425863DPR
CSB-MP425863DPR
CSB-EP425863DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Phosphoglucosamine mutase (glmM) CSB-YP425864DPR
CSB-EP425864DPR
CSB-BP425864DPR
CSB-MP425864DPR
CSB-EP425864DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans 30S ribosomal protein S18 (rpsR) CSB-YP425865DPR
CSB-EP425865DPR
CSB-BP425865DPR
CSB-MP425865DPR
CSB-EP425865DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP425866DPR
CSB-EP425866DPR
CSB-BP425866DPR
CSB-MP425866DPR
CSB-EP425866DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii S-adenosylmethionine synthase (METM) CSB-YP425867DSJ
CSB-EP425867DSJ
CSB-BP425867DSJ
CSB-MP425867DSJ
CSB-EP425867DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP425868DPR
CSB-EP425868DPR
CSB-BP425868DPR
CSB-MP425868DPR
CSB-EP425868DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Transcriptional repressor NrdR (nrdR) CSB-YP425869DPR
CSB-EP425869DPR
CSB-BP425869DPR
CSB-MP425869DPR
CSB-EP425869DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Adenylosuccinate synthetase (purA) CSB-YP425870DPR
CSB-EP425870DPR
CSB-BP425870DPR
CSB-MP425870DPR
CSB-EP425870DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP425871DPR
CSB-EP425871DPR
CSB-BP425871DPR
CSB-MP425871DPR
CSB-EP425871DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP425872DPR
CSB-EP425872DPR
CSB-BP425872DPR
CSB-MP425872DPR
CSB-EP425872DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP425873DPR
CSB-EP425873DPR
CSB-BP425873DPR
CSB-MP425873DPR
CSB-EP425873DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP425874DPR
CSB-EP425874DPR
CSB-BP425874DPR
CSB-MP425874DPR
CSB-EP425874DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Serine--tRNA ligase (serS) CSB-YP425875DPR
CSB-EP425875DPR
CSB-BP425875DPR
CSB-MP425875DPR
CSB-EP425875DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Bifunctional protein HldE (hldE) CSB-YP425876DPR
CSB-EP425876DPR
CSB-BP425876DPR
CSB-MP425876DPR
CSB-EP425876DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP425877DPR
CSB-EP425877DPR
CSB-BP425877DPR
CSB-MP425877DPR
CSB-EP425877DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Exodeoxyribonuclease 7 large subunit (xseA), partial CSB-YP425878DPR
CSB-EP425878DPR
CSB-BP425878DPR
CSB-MP425878DPR
CSB-EP425878DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>