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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Danio rerio Serine/threonine-protein phosphatase 4 catalytic subunit A (ppp4ca) CSB-YP427600DIL
CSB-EP427600DIL
CSB-BP427600DIL
CSB-MP427600DIL
CSB-EP427600DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Lysosomal protein NCU-G1-B, partial CSB-YP427601XBE
CSB-EP427601XBE
CSB-BP427601XBE
CSB-MP427601XBE
CSB-EP427601XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant APOPT family protein CBG23705, mitochondrial (CBG23705), partial CSB-YP427602CXX
CSB-EP427602CXX
CSB-BP427602CXX
CSB-MP427602CXX
CSB-EP427602CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative UDP-glucuronosyltransferase ugt-47 (ugt-47), partial CSB-YP427603CXX1
CSB-EP427603CXX1
CSB-BP427603CXX1
CSB-MP427603CXX1
CSB-EP427603CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine-rich membrane protein KE4 homolog 1 (hke-4.1), partial CSB-YP427604CXX1
CSB-EP427604CXX1
CSB-BP427604CXX1
CSB-MP427604CXX1
CSB-EP427604CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATPase asna-1 (asna-1) CSB-YP427605CXX
CSB-EP427605CXX
CSB-BP427605CXX
CSB-MP427605CXX
CSB-EP427605CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit 1, mitochondrial (clpp-1) CSB-YP427606CXX
CSB-EP427606CXX
CSB-BP427606CXX
CSB-MP427606CXX
CSB-EP427606CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleotide 5'-hydroxyl-kinase nol-9 (nol-9), partial CSB-YP427607CXX
CSB-EP427607CXX
CSB-BP427607CXX
CSB-MP427607CXX
CSB-EP427607CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0729 protein CBG02799 (CBG02799) CSB-YP427608CXX
CSB-EP427608CXX
CSB-BP427608CXX
CSB-MP427608CXX
CSB-EP427608CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial (CBG02769), partial CSB-YP427609CXX
CSB-EP427609CXX
CSB-BP427609CXX
CSB-MP427609CXX
CSB-EP427609CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant C-type lectin domain-containing protein 87 (clec-87) CSB-YP427610CXX
CSB-EP427610CXX
CSB-BP427610CXX
CSB-MP427610CXX
CSB-EP427610CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rho-associated protein kinase let-502 (let-502), partial CSB-YP427611CXX
CSB-EP427611CXX
CSB-BP427611CXX
CSB-MP427611CXX
CSB-EP427611CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein pop-1 (pop-1) CSB-YP427612CXX
CSB-EP427612CXX
CSB-BP427612CXX
CSB-MP427612CXX
CSB-EP427612CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0533 protein CBG04321 (CBG04321) CSB-YP427613CXX
CSB-EP427613CXX
CSB-BP427613CXX
CSB-MP427613CXX
CSB-EP427613CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine/threonine-protein kinase par-4 (par-4), partial CSB-YP427614CXX
CSB-EP427614CXX
CSB-BP427614CXX
CSB-MP427614CXX
CSB-EP427614CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent (S)-NAD (P)H-hydrate dehydratase CSB-YP427615CXX
CSB-EP427615CXX
CSB-BP427615CXX
CSB-MP427615CXX
CSB-EP427615CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Integrin alpha pat-2 (pat-2), partial CSB-YP427616CXX
CSB-EP427616CXX
CSB-BP427616CXX
CSB-MP427616CXX
CSB-EP427616CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trehalose-phosphatase (gob-1) CSB-YP427617CXX
CSB-EP427617CXX
CSB-BP427617CXX
CSB-MP427617CXX
CSB-EP427617CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant cGMP-dependent protein kinase egl-4 (egl-4), partial CSB-YP427618CXX
CSB-EP427618CXX
CSB-BP427618CXX
CSB-MP427618CXX
CSB-EP427618CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein KIAA0664 homolog (clu-1), partial CSB-YP427619CXX
CSB-EP427619CXX
CSB-BP427619CXX
CSB-MP427619CXX
CSB-EP427619CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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