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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dinoroseobacter shibae 50S ribosomal protein L19 (rplS) CSB-YP425939DIB
CSB-EP425939DIB
CSB-BP425939DIB
CSB-MP425939DIB
CSB-EP425939DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP425940DIB
CSB-EP425940DIB
CSB-BP425940DIB
CSB-MP425940DIB
CSB-EP425940DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Arginine deiminase (arcA) CSB-YP425941DIB
CSB-EP425941DIB
CSB-BP425941DIB
CSB-MP425941DIB
CSB-EP425941DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae UPF0246 protein Dshi_3333 (Dshi_3333) CSB-YP425942DIB
CSB-EP425942DIB
CSB-BP425942DIB
CSB-MP425942DIB
CSB-EP425942DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP425943DIB
CSB-EP425943DIB
CSB-BP425943DIB
CSB-MP425943DIB
CSB-EP425943DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP425944DIB
CSB-EP425944DIB
CSB-BP425944DIB
CSB-MP425944DIB
CSB-EP425944DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Ribosome maturation factor RimP (rimP) CSB-YP425945DIB
CSB-EP425945DIB
CSB-BP425945DIB
CSB-MP425945DIB
CSB-EP425945DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Phosphoglycerate kinase (pgk) CSB-YP425946DIB
CSB-EP425946DIB
CSB-BP425946DIB
CSB-MP425946DIB
CSB-EP425946DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Undecaprenyl-diphosphatase (uppP), partial CSB-YP425947DIB1
CSB-EP425947DIB1
CSB-BP425947DIB1
CSB-MP425947DIB1
CSB-EP425947DIB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Urease accessory protein UreD (ureD) CSB-YP425948DIB
CSB-EP425948DIB
CSB-BP425948DIB
CSB-MP425948DIB
CSB-EP425948DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Peptidyl-tRNA hydrolase (pth) CSB-YP425949DIB
CSB-EP425949DIB
CSB-BP425949DIB
CSB-MP425949DIB
CSB-EP425949DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Glycine--tRNA ligase beta subunit (glyS) CSB-YP425950DIB
CSB-EP425950DIB
CSB-BP425950DIB
CSB-MP425950DIB
CSB-EP425950DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae L-rhamnose mutarotase (rhaM) CSB-YP425951DIB
CSB-EP425951DIB
CSB-BP425951DIB
CSB-MP425951DIB
CSB-EP425951DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Cytidylate kinase (cmk) CSB-YP425952DIB
CSB-EP425952DIB
CSB-BP425952DIB
CSB-MP425952DIB
CSB-EP425952DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola UPF0042 nucleotide-binding protein Sare_3328 (Sare_3328) CSB-YP425953STC
CSB-EP425953STC
CSB-BP425953STC
CSB-MP425953STC
CSB-EP425953STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Probable transcriptional regulatory protein Sare_1779 (Sare_1779) CSB-YP425954STC
CSB-EP425954STC
CSB-BP425954STC
CSB-MP425954STC
CSB-EP425954STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP425955STC
CSB-EP425955STC
CSB-BP425955STC
CSB-MP425955STC
CSB-EP425955STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP425956STC
CSB-EP425956STC
CSB-BP425956STC
CSB-MP425956STC
CSB-EP425956STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Alanine--tRNA ligase (alaS), partial CSB-YP425957STC
CSB-EP425957STC
CSB-BP425957STC
CSB-MP425957STC
CSB-EP425957STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP425958STC
CSB-EP425958STC
CSB-BP425958STC
CSB-MP425958STC
CSB-EP425958STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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