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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salinispora arenicola 50S ribosomal protein L35 (rpmI) CSB-YP425959STC
CSB-EP425959STC
CSB-BP425959STC
CSB-MP425959STC
CSB-EP425959STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Octanoyltransferase (lipB) CSB-YP425960STC
CSB-EP425960STC
CSB-BP425960STC
CSB-MP425960STC
CSB-EP425960STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Glutamate--tRNA ligase (gltX) CSB-YP425961STC
CSB-EP425961STC
CSB-BP425961STC
CSB-MP425961STC
CSB-EP425961STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Glutamate 5-kinase (proB) CSB-YP425962STC
CSB-EP425962STC
CSB-BP425962STC
CSB-MP425962STC
CSB-EP425962STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola 50S ribosomal protein L21 (rplU) CSB-YP425963STC
CSB-EP425963STC
CSB-BP425963STC
CSB-MP425963STC
CSB-EP425963STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP425964STC
CSB-EP425964STC
CSB-BP425964STC
CSB-MP425964STC
CSB-EP425964STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola Proteasome subunit alpha (prcA) CSB-YP425965STC
CSB-EP425965STC
CSB-BP425965STC
CSB-MP425965STC
CSB-EP425965STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora arenicola ATP synthase gamma chain (atpG) CSB-YP425966STC
CSB-EP425966STC
CSB-BP425966STC
CSB-MP425966STC
CSB-EP425966STC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea DNA-directed RNA polymerase subunit beta' (rpoC1), partial CSB-YP425967IND
CSB-EP425967IND
CSB-BP425967IND
CSB-MP425967IND
CSB-EP425967IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP425968IND
CSB-EP425968IND
CSB-BP425968IND
CSB-MP425968IND
CSB-EP425968IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea Apocytochrome f (petA), partial CSB-YP425969IND1
CSB-EP425969IND1
CSB-BP425969IND1
CSB-MP425969IND1
CSB-EP425969IND1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea Protein ycf2 (ycf2-A), partial CSB-YP425970IND
CSB-EP425970IND
CSB-BP425970IND
CSB-MP425970IND
CSB-EP425970IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea purpurea Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP425971IND
CSB-EP425971IND
CSB-BP425971IND
CSB-MP425971IND
CSB-EP425971IND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine ATPase family AAA domain-containing protein 3 (ATAD3), partial CSB-YP425972BO1
CSB-EP425972BO1
CSB-BP425972BO1
CSB-MP425972BO1
CSB-EP425972BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens DNA replication and repair protein recF (recF) CSB-YP425973BQD
CSB-EP425973BQD
CSB-BP425973BQD
CSB-MP425973BQD
CSB-EP425973BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP425974BQD
CSB-EP425974BQD
CSB-BP425974BQD
CSB-MP425974BQD
CSB-EP425974BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 50S ribosomal protein L14 (rplN) CSB-YP425975BQD
CSB-EP425975BQD
CSB-BP425975BQD
CSB-MP425975BQD
CSB-EP425975BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 50S ribosomal protein L15 (rplO) CSB-YP425976BQD
CSB-EP425976BQD
CSB-BP425976BQD
CSB-MP425976BQD
CSB-EP425976BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 50S ribosomal protein L17 (rplQ) CSB-YP425977BQD
CSB-EP425977BQD
CSB-BP425977BQD
CSB-MP425977BQD
CSB-EP425977BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens NH (3)-dependent NAD (+) synthetase CSB-YP425978BQD
CSB-EP425978BQD
CSB-BP425978BQD
CSB-MP425978BQD
CSB-EP425978BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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