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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O9:H4 Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP426159EJF
CSB-EP426159EJF
CSB-BP426159EJF
CSB-MP426159EJF
CSB-EP426159EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Ribosomal protein S6 modification protein (rimK) CSB-YP426160EJF
CSB-EP426160EJF
CSB-BP426160EJF
CSB-MP426160EJF
CSB-EP426160EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Serine--tRNA ligase (serS) CSB-YP426161EJF
CSB-EP426161EJF
CSB-BP426161EJF
CSB-MP426161EJF
CSB-EP426161EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP426162EJF
CSB-EP426162EJF
CSB-BP426162EJF
CSB-MP426162EJF
CSB-EP426162EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP426163EJF
CSB-EP426163EJF
CSB-BP426163EJF
CSB-MP426163EJF
CSB-EP426163EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Chaperone modulatory protein CbpM (cbpM) CSB-YP426164EJF
CSB-EP426164EJF
CSB-BP426164EJF
CSB-MP426164EJF
CSB-EP426164EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glyoxylate/hydroxypyruvate reductase A (ghrA) CSB-YP426165EJF
CSB-EP426165EJF
CSB-BP426165EJF
CSB-MP426165EJF
CSB-EP426165EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Dihydroorotase (pyrC) CSB-YP426166EJF
CSB-EP426166EJF
CSB-BP426166EJF
CSB-MP426166EJF
CSB-EP426166EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP426167EJF
CSB-EP426167EJF
CSB-BP426167EJF
CSB-MP426167EJF
CSB-EP426167EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Fatty acid metabolism regulator protein (fadR) CSB-YP426168EJF
CSB-EP426168EJF
CSB-BP426168EJF
CSB-MP426168EJF
CSB-EP426168EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP426169EJF
CSB-EP426169EJF
CSB-BP426169EJF
CSB-MP426169EJF
CSB-EP426169EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Tryptophan synthase beta chain (trpB) CSB-YP426170EJF
CSB-EP426170EJF
CSB-BP426170EJF
CSB-MP426170EJF
CSB-EP426170EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glucans biosynthesis protein D (mdoD) CSB-YP426171EJF
CSB-EP426171EJF
CSB-BP426171EJF
CSB-MP426171EJF
CSB-EP426171EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Autoinducer 2 import system permease protein lsrD (lsrD), partial CSB-YP426172EJF
CSB-EP426172EJF
CSB-BP426172EJF
CSB-MP426172EJF
CSB-EP426172EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Electron transport complex protein RnfC (rnfC) CSB-YP426173EJF
CSB-EP426173EJF
CSB-BP426173EJF
CSB-MP426173EJF
CSB-EP426173EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Ribonuclease T (rnt) CSB-YP426174EJF
CSB-EP426174EJF
CSB-BP426174EJF
CSB-MP426174EJF
CSB-EP426174EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0061 protein ydiU (ydiU) CSB-YP426175EJF
CSB-EP426175EJF
CSB-BP426175EJF
CSB-MP426175EJF
CSB-EP426175EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Threonine--tRNA ligase (thrS) CSB-YP426176EJF
CSB-EP426176EJF
CSB-BP426176EJF
CSB-MP426176EJF
CSB-EP426176EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Succinylornithine transaminase (astC) CSB-YP426177EJF
CSB-EP426177EJF
CSB-BP426177EJF
CSB-MP426177EJF
CSB-EP426177EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Leucine efflux protein (leuE), partial CSB-YP426178EJF1
CSB-EP426178EJF1
CSB-BP426178EJF1
CSB-MP426178EJF1
CSB-EP426178EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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