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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Serratia proteamaculans G/U mismatch-specific DNA glycosylase (mug) CSB-YP426719STJ
CSB-EP426719STJ
CSB-BP426719STJ
CSB-MP426719STJ
CSB-EP426719STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L13 (rplM) CSB-YP426720STJ
CSB-EP426720STJ
CSB-BP426720STJ
CSB-MP426720STJ
CSB-EP426720STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein AaeX (aaeX), partial CSB-YP426721STJ1
CSB-EP426721STJ1
CSB-BP426721STJ1
CSB-MP426721STJ1
CSB-EP426721STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 4-hydroxybenzoate octaprenyltransferase (ubiA) CSB-YP426722STJ
CSB-EP426722STJ
CSB-BP426722STJ
CSB-MP426722STJ
CSB-EP426722STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Homoserine O-succinyltransferase (metA) CSB-YP426723STJ
CSB-EP426723STJ
CSB-BP426723STJ
CSB-MP426723STJ
CSB-EP426723STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 30S ribosomal protein S13 (rpsM) CSB-YP426724STJ
CSB-EP426724STJ
CSB-BP426724STJ
CSB-MP426724STJ
CSB-EP426724STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L6 (rplF) CSB-YP426725STJ
CSB-EP426725STJ
CSB-BP426725STJ
CSB-MP426725STJ
CSB-EP426725STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L22 (rplV) CSB-YP426726STJ
CSB-EP426726STJ
CSB-BP426726STJ
CSB-MP426726STJ
CSB-EP426726STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein TusC (tusC) CSB-YP426727STJ
CSB-EP426727STJ
CSB-BP426727STJ
CSB-MP426727STJ
CSB-EP426727STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Ferrous iron transport protein C (feoC) CSB-YP426728STJ
CSB-EP426728STJ
CSB-BP426728STJ
CSB-MP426728STJ
CSB-EP426728STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Glycogen synthase (glgA) CSB-YP426729STJ
CSB-EP426729STJ
CSB-BP426729STJ
CSB-MP426729STJ
CSB-EP426729STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0261 protein Spro_4740 (Spro_4740) CSB-YP426730STJ
CSB-EP426730STJ
CSB-BP426730STJ
CSB-MP426730STJ
CSB-EP426730STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans tRNA (uracil (54)-C (5))-methyltransferase CSB-YP426731STJ
CSB-EP426731STJ
CSB-BP426731STJ
CSB-MP426731STJ
CSB-EP426731STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP426732STJ
CSB-EP426732STJ
CSB-BP426732STJ
CSB-MP426732STJ
CSB-EP426732STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Cation-efflux pump FieF (fieF), partial CSB-YP426733STJ1
CSB-EP426733STJ1
CSB-BP426733STJ1
CSB-MP426733STJ1
CSB-EP426733STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP426734STJ
CSB-EP426734STJ
CSB-BP426734STJ
CSB-MP426734STJ
CSB-EP426734STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Orotate phosphoribosyltransferase (pyrE) CSB-YP426735STJ
CSB-EP426735STJ
CSB-BP426735STJ
CSB-MP426735STJ
CSB-EP426735STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP426736STJ
CSB-EP426736STJ
CSB-BP426736STJ
CSB-MP426736STJ
CSB-EP426736STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari Putative phosphotransferase A1C_00005 (A1C_00005) CSB-YP426737RIL
CSB-EP426737RIL
CSB-BP426737RIL
CSB-MP426737RIL
CSB-EP426737RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia akari tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP426738RIL
CSB-EP426738RIL
CSB-BP426738RIL
CSB-MP426738RIL
CSB-EP426738RIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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