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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Serratia proteamaculans 50S ribosomal protein L27 (rpmA) CSB-YP426659STJ
CSB-EP426659STJ
CSB-BP426659STJ
CSB-MP426659STJ
CSB-EP426659STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP426660STJ
CSB-EP426660STJ
CSB-BP426660STJ
CSB-MP426660STJ
CSB-EP426660STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Trp operon repressor (trpR) CSB-YP426661STJ
CSB-EP426661STJ
CSB-BP426661STJ
CSB-MP426661STJ
CSB-EP426661STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Chaperone protein DnaK (dnaK), partial CSB-YP426662STJ
CSB-EP426662STJ
CSB-BP426662STJ
CSB-MP426662STJ
CSB-EP426662STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP426663STJ
CSB-EP426663STJ
CSB-BP426663STJ
CSB-MP426663STJ
CSB-EP426663STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP426664STJ
CSB-EP426664STJ
CSB-BP426664STJ
CSB-MP426664STJ
CSB-EP426664STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP426665STJ
CSB-EP426665STJ
CSB-BP426665STJ
CSB-MP426665STJ
CSB-EP426665STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP426666STJ
CSB-EP426666STJ
CSB-BP426666STJ
CSB-MP426666STJ
CSB-EP426666STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Glutamate--cysteine ligase (gshA), partial CSB-YP426667STJ
CSB-EP426667STJ
CSB-BP426667STJ
CSB-MP426667STJ
CSB-EP426667STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 50S ribosomal protein L19 (rplS) CSB-YP426668STJ
CSB-EP426668STJ
CSB-BP426668STJ
CSB-MP426668STJ
CSB-EP426668STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Acireductone dioxygenase (mtnD) CSB-YP426669STJ
CSB-EP426669STJ
CSB-BP426669STJ
CSB-MP426669STJ
CSB-EP426669STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Lipid A palmitoyltransferase PagP (pagP) CSB-YP426670STJ
CSB-EP426670STJ
CSB-BP426670STJ
CSB-MP426670STJ
CSB-EP426670STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans N utilization substance protein B homolog (nusB) CSB-YP426671STJ
CSB-EP426671STJ
CSB-BP426671STJ
CSB-MP426671STJ
CSB-EP426671STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Trigger factor (tig) CSB-YP426672STJ
CSB-EP426672STJ
CSB-BP426672STJ
CSB-MP426672STJ
CSB-EP426672STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Recombination protein RecR (recR) CSB-YP426673STJ
CSB-EP426673STJ
CSB-BP426673STJ
CSB-MP426673STJ
CSB-EP426673STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein CrcB homolog (crcB), partial CSB-YP426674STJ1
CSB-EP426674STJ1
CSB-BP426674STJ1
CSB-MP426674STJ1
CSB-EP426674STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Probable rRNA maturation factor (Spro_1217) CSB-YP426675STJ
CSB-EP426675STJ
CSB-BP426675STJ
CSB-MP426675STJ
CSB-EP426675STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Potassium-transporting ATPase A chain (kdpA), partial CSB-YP426676STJ
CSB-EP426676STJ
CSB-BP426676STJ
CSB-MP426676STJ
CSB-EP426676STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans DNA protection during starvation protein (dps) CSB-YP426677STJ
CSB-EP426677STJ
CSB-BP426677STJ
CSB-MP426677STJ
CSB-EP426677STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Cyanate hydratase (cynS) CSB-YP426678STJ
CSB-EP426678STJ
CSB-BP426678STJ
CSB-MP426678STJ
CSB-EP426678STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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