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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Undecaprenyl-diphosphatase (uppP), partial CSB-YP427720SUM
CSB-EP427720SUM
CSB-BP427720SUM
CSB-MP427720SUM
CSB-EP427720SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP427721SUM
CSB-EP427721SUM
CSB-BP427721SUM
CSB-MP427721SUM
CSB-EP427721SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0042 nucleotide-binding protein USA300HOU_0794 (USA300HOU_0794) CSB-YP427722SUM
CSB-EP427722SUM
CSB-BP427722SUM
CSB-MP427722SUM
CSB-EP427722SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit F1, partial CSB-YP427723SUM1
CSB-EP427723SUM1
CSB-BP427723SUM1
CSB-MP427723SUM1
CSB-EP427723SUM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit C1, partial CSB-YP427724SUM1
CSB-EP427724SUM1
CSB-BP427724SUM1
CSB-MP427724SUM1
CSB-EP427724SUM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP427725SUM
CSB-EP427725SUM
CSB-BP427725SUM
CSB-MP427725SUM
CSB-EP427725SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0344 protein USA300HOU_0928 (USA300HOU_0928), partial CSB-YP427726SUM1
CSB-EP427726SUM1
CSB-BP427726SUM1
CSB-MP427726SUM1
CSB-EP427726SUM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP427727SUM
CSB-EP427727SUM
CSB-BP427727SUM
CSB-MP427727SUM
CSB-EP427727SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus PTS system glucose-specific EIICBA component (ptsG), partial CSB-YP427728SUM
CSB-EP427728SUM
CSB-BP427728SUM
CSB-MP427728SUM
CSB-EP427728SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus L-lactate dehydrogenase 1 (ldh1) CSB-YP427729SUM
CSB-EP427729SUM
CSB-BP427729SUM
CSB-MP427729SUM
CSB-EP427729SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Xanthine phosphoribosyltransferase (xpt) CSB-YP427730SUM
CSB-EP427730SUM
CSB-BP427730SUM
CSB-MP427730SUM
CSB-EP427730SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0753 protein USA300HOU_0458 (USA300HOU_0458), partial CSB-YP427731SUM
CSB-EP427731SUM
CSB-BP427731SUM
CSB-MP427731SUM
CSB-EP427731SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhA2 (mnhA2), partial CSB-YP427732SUM
CSB-EP427732SUM
CSB-BP427732SUM
CSB-MP427732SUM
CSB-EP427732SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhD2 (mnhD2), partial CSB-YP427733SUM
CSB-EP427733SUM
CSB-BP427733SUM
CSB-MP427733SUM
CSB-EP427733SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhG2 (mnhG2), partial CSB-YP427734SUM1
CSB-EP427734SUM1
CSB-BP427734SUM1
CSB-MP427734SUM1
CSB-EP427734SUM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0349 protein USA300HOU_0900 (USA300HOU_0900) CSB-YP427735SUM
CSB-EP427735SUM
CSB-BP427735SUM
CSB-MP427735SUM
CSB-EP427735SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP427736SUM
CSB-EP427736SUM
CSB-BP427736SUM
CSB-MP427736SUM
CSB-EP427736SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heme A synthase (ctaA), partial CSB-YP427737SUM
CSB-EP427737SUM
CSB-BP427737SUM
CSB-MP427737SUM
CSB-EP427737SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0348 protein USA300HOU_1063 (USA300HOU_1063) CSB-YP427738SUM
CSB-EP427738SUM
CSB-BP427738SUM
CSB-MP427738SUM
CSB-EP427738SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus MutS2 protein (mutS2), partial CSB-YP427739SUM
CSB-EP427739SUM
CSB-BP427739SUM
CSB-MP427739SUM
CSB-EP427739SUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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